Invitrogen

ADCK5 Polyclonal Antibody

查看其他3个ADCK5抗体

抗体检测数据 (3)

ADCK5 Antibody in Immunocytochemistry (ICC/IF)

ADCK5 Antibody in Immunocytochemistry (ICC/IF)

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ADCK5 Antibody (PA5-106505) in ICC/IF

Immunocytochemistry analysis of ADCK5 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ADCK5 polyclonal antibody (Product # PA5-106505) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

ADCK5 Antibody in Immunocytochemistry (ICC/IF)

ADCK5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ADCK5 Antibody in Western Blot (WB)

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ADCK5 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ADCK5 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ADCK5 polyclonal antibody (Product # PA5-106505) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

ADCK5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ADCK5 in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with ADCK5 polyclonal antibody (Product # PA5-106505) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

ADCK5 Antibody in Western Blot (WB) — Western blot analysis of ADCK5 in mouse lung. Samples were incubated with ADCK5 polyclonal antibody (Product # PA5-106505).

产品信息

PA5-106505

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human ADCK5(Accession Q3MIX3), corresponding to amino acid residues P45-L95.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2854174

产品详细信息

Antibody detects endogenous levels of total ADCK5.

靶标信息

ADCK5 is member of the AarF domain containing kinase family.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Uncharacterized aarF domain-containing protein kinase 5

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基因别名: A230108P17; ADCK5

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UniProt ID: (Human) Q3MIX3, (Mouse) Q80V03

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Entrez Gene ID: (Human) 203054, (Mouse) 268822

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protein serine/threonine kinase activity protein binding molecular_function transmembrane receptor protein serine/threonine kinase activity 3-phosphoinositide-dependent protein kinase activity DNA-dependent protein kinase activity AMP-activated protein kinase activity cyclic nucleotide-dependent protein kinase activity eukaryotic translation initiation factor 2alpha kinase activity calcium-dependent protein kinase C activity receptor signaling protein serine/threonine kinase activity NF-kappaB-inducing kinase activity JUN kinase activity JUN kinase kinase kinase activity MAP kinase kinase activity MAP kinase kinase kinase activity ribosomal protein S6 kinase activity MAP kinase kinase kinase kinase activity JUN kinase kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SAP kinase activity transmembrane receptor protein kinase activity cyclin-dependent protein kinase activating kinase activity GTP-dependent protein kinase activity ATP-binding cassette (ABC) transporter primary active transporter transporter

参与通路

protein phosphorylation biological_process phosphorylation