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ADORA1 Antibody (PA1-041A) in ICC/IF
Immunofluorescence analysis of Adenosine Receptor A1 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (Product # PA1-041A) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 48... View More
产品信息
PA1-041A
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2222233
产品详细信息
PA1-041A detects adenosine receptor A1 (A1AR) in rat tissues. This antibody does not detect other AR subtypes. Previous lots of this antibody have been used in human and bovine samples.
PA1-041A has been successfully used in Immunohistochemistry (paraffin) and Western blot procedures. By Western blot, this antibody detects an ~37 kDa protein in rat brain representing A1AR.
The PA1-041A immunogen is a synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.
靶标信息
Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: adenosine A1 receptor variant 1; adenosine A1 receptor variant 2; Adenosine receptor A1; ADO-A1 receptor
基因别名: ADORA1; RDC7
UniProt ID: (Human) P30542, (Rat) P25099
Entrez Gene ID: (Human) 134, (Rat) 29290
G-protein coupled adenosine receptor activity
G-protein coupled receptor binding
purine nucleoside binding
phospholipase C activity
protein binding
heat shock protein binding
G-protein beta/gamma-subunit complex binding
heterotrimeric G-protein binding
protein heterodimerization activity
G-protein coupled receptor
activation of MAPKK activity
temperature homeostasis
response to hypoxia
adenosine receptor signaling pathway
regulation of respiratory gaseous exchange by neurological system process
negative regulation of acute inflammatory response
negative regulation of leukocyte migration
positive regulation of peptide secretion
regulation of glomerular filtration
protein targeting to membrane
phagocytosis
inflammatory response
signal transduction
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
cell-cell signaling
nervous system development
negative regulation of cell proliferation
negative regulation of glutamate secretion
lipid catabolic process
negative regulation of synaptic transmission, GABAergic
positive regulation of nucleoside transport
negative regulation of neurotrophin production
positive regulation of protein dephosphorylation
negative regulation of renal sodium excretion
negative regulation of circadian sleep/wake cycle, non-REM sleep
negative regulation of apoptotic process
positive regulation of potassium ion transport
positive regulation of epidermal growth factor-activated receptor activity
negative regulation of blood pressure
positive regulation of blood pressure
negative regulation of vasodilation
negative regulation of hormone secretion
cognition
detection of temperature stimulus involved in sensory perception of pain
negative regulation of lipid catabolic process
regulation of sensory perception of pain
negative regulation of synaptic transmission, glutamatergic
negative regulation of cardiac muscle contraction
excitatory postsynaptic potential
relaxation of vascular smooth muscle
negative regulation of mucus secretion
apoptotic signaling pathway
negative regulation of long term synaptic depression
G-protein coupled receptor signaling pathway
regulation of vasodilation
negative regulation of circadian sleep/wake cycle, sleep
negative regulation of heart contraction
regulation of synaptic plasticity
negative regulation of inflammatory response
regulation of cardiac muscle contraction
