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Invitrogen

ADORA2A Polyclonal Antibody

1个已发表图片

9 篇参考文献

查看其他17个ADORA2A抗体

说明书

实验方案

技术支持

货号 PA1-042

CNY 5,798.00

100 µL

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说明书

实验方案

技术支持

抗体检测数据 (5)
已发表图片 (1)

Application

ADORA2A Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

ADORA2A Antibody (PA1-042) in ICC/IF

Immunofluorescent analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Adenosine Receptor A2a polyclonal antibody (Product # PA1-042) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and... View More

ADORA2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA1-042

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫细胞化学 (ICC/IF)

1:10-1:100

免疫沉淀 (IP)

Assay-dependent

in situ PLA (PLA)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

Dog, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.69 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2257858

产品详细信息

PA1-042 detects adenosine receptor A2a (A2aAR) in human, rat, mouse and canine tissues. This antibody does not detect other AR subtypes.

PA1-042 has been successfully used in Western blot, ICC/IF, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~45 kDa protein from canine striatum representing A2aAR. Immunohistochemical staining of A2aAR in human hippocampus with PA1-042 yields a pattern consistent with plasma membrane staining.

The PA1-042 immunogen is a synthetic peptide corresponding to residues E(373) S H G D M G L P D V E L L S H E L K(391) of canine A2aAR.

靶标信息

ADORA2A is a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. This protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Adenosine Receptors Expression in Human Retina and Choroid with Age-related Macular Degeneration.

Journal of ophthalmic & vision research

Goebel CP,Song YS,Zaitoun IS,Wang S,Potter HAD,Sorenson CM,Sheibani N

PA1-042 was used in Immunohistochemistry to find that all four receptor subtypes were expressed in several layers of the retina, and within the retinal pigment epithelium and choroid.

Sat Jun 10 00:00:00 UTC 2023

Adenosine receptors differentially mediate enteric glial cell death induced by Clostridioides difficile Toxins A and B.

Frontiers in immunology

Costa DVS,Shin JH,Goldbeck SM,Bolick DT,Mesquita FS,Loureiro AV,Rodrigues-Jesus MJ,Brito GAC,Warren CA

PA1-042 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that adenosine receptors appear to play a regulatory role in EGCs death and proinflammatory response induced by TcdA and TcdB, and thus may be potential targets of intervention to prevent post-CDI intestinal dysmotility.

Fri Feb 03 00:00:00 UTC 2023

Cell-type specific profiling of histone post-translational modifications in the adult mouse striatum.

Nature communications

Carpenter MD,Fischer DK,Zhang S,Bond AM,Czarnecki KS,Woolf MT,Song H,Heller EA

PA1-042 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to understand combinatorial relationships between neuronal-subtype-specific epigenetic modifications and gene expression.

Tue Dec 13 00:00:00 UTC 2022

A venous-specific purinergic signaling cascade initiated by Pannexin 1 regulates TNFα-induced increases in endothelial permeability.

Science signaling

Maier-Begandt D,Comstra HS,Molina SA,Krüger N,Ruddiman CA,Chen YL,Chen X,Biwer LA,Johnstone SR,Lohman AW,Good ME,DeLalio LJ,Hong K,Bacon HM,Yan Z,Sonkusare SK,Koval M,Isakson BE

PA1-042 was used in Immunohistochemistry to provide a targetable pathway with the potential to promote vein barrier function and prevent the deleterious effects of vascular leak in response to inflammation.

Tue Mar 02 00:00:00 UTC 2021

Methamphetamine Blocks Adenosine A_2A Receptor Activation via Sigma 1 and Cannabinoid CB₁ Receptors.

International journal of molecular sciences

Casanovas M,Reyes-Resina I,Lillo A,Lillo J,López-Arnau R,Camarasa J,Escubedo E,Navarro G,Franco R

PA1-042 was used in Proximity Ligation Assay (PLA) to investigate the impact of methamphetamine on adenosine A2A receptor (A2AR) and cannabinoid CB1 receptor (CB1R) complexes.

Tue Mar 09 00:00:00 UTC 2021

On the Role of Adenosine A2A Receptor Gene Transcriptional Regulation in Parkinson's Disease.

Frontiers in neuroscience

Falconi A,Bonito-Oliva A,Di Bartolomeo M,Massimini M,Fattapposta F,Locuratolo N,Dainese E,Pascale E,Fisone G,D'Addario C

Published figure using ADORA2A polyclonal antibody (Product # PA1-042) in Western Blot

Wed Sep 30 00:00:00 UTC 2020

Abnormal calcium handling in atrial fibrillation is linked to up-regulation of adenosine A2A receptors.

European heart journal

Llach A,Molina CE,Prat-Vidal C,Fernandes J,Casadó V,Ciruela F,Lluís C,Franco R,Cinca J,Hove-Madsen L

PA1-042 was used in western blot to investigate the relationship between calcium signaling and adenosine A receptor level in atrial fibrillation

Tue Mar 01 00:00:00 UTC 2011

Adenosine A 2B receptors modulate cAMP levels and induce CREB but not ERK1/2 and p38 phosphorylation in rat skeletal muscle cells.

Biochemical and biophysical research communications

Lynge J,Schulte G,Nordsborg N,Fredholm BB,Hellsten Y

PA1-042 was used in western blot to investigate the role of adenosine A(2B) on specific signal pathway in rat skeletal muscle cells

Fri Jul 18 00:00:00 UTC 2003

Stimulation of A(2A) adenosine receptor phosphorylation by protein kinase C activation: evidence for regulation by multiple protein kinase C isoforms.

Biochemistry

Palmer TM,Stiles GL

PA1-042 was used in western blot to investigate the mechanism of A(2A) adenosine receptor phosphorylation by protein kinase C activation

Tue Nov 09 00:00:00 UTC 1999

生物信息学

蛋白别名: A2a receptor; Adenosine A2a-receptor; Adenosine receptor A2a; adenosine receptor subtype A2a; AR; hA2aR; unnamed protein product

基因别名: A2AAR; A2aR; AA2AR; ADENO; ADORA2; ADORA2A; Adora2l1; RDC8

UniProt ID: (Human) P29274, (Dog) P11617, (Mouse) Q60613, (Rat) P30543

Entrez Gene ID: (Human) 135, (Dog) 403960, (Mouse) 11540, (Rat) 25369

功能

G-protein coupled adenosine receptor activity protein binding enzyme binding identical protein binding signal transducer activity G-protein coupled receptor activity type 5 metabotropic glutamate receptor binding protein heterodimerization activity alpha-actinin binding G-protein coupled receptor

参与通路

ADORA2A Polyclonal Antibody

ADORA2A Antibody (PA1-042) in ICC/IF

产品信息

PA1-042

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

in situ PLA (PLA)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

ADORA2A Polyclonal Antibody

ADORA2A Antibody (PA1-042) in ICC/IF

产品信息

PA1-042

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

in situ PLA (PLA)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID