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Invitrogen
AFAP1L2 Polyclonal Antibody
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FIGURE: 1 / 3
AFAP1L2 Antibody (PA5-100378) in ICC/IF
Immunofluorescent analysis of AFAP1L2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and AFAP1L2 polyclonal antibody (Product # PA5-100378) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100378
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AFAP1L2(Accession Q8N4X5), corresponding to amino acid residues N67-T117.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total AFAP1L2.
Target Information
AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin filament associated protein 1-like 2; Actin filament-associated protein 1-like 2; AFAP1-like protein 2; CTB-1144G6.6; phosphatidylinositol 3-kinase-associated protein
Gene Aliases: AFAP1L2; AU041783; C86904; CTB-1144G6.4; KIAA1914; mKIAA1914; RGD1310668; XB130
UniProt ID: (Human) Q8N4X5, (Mouse) Q5DTU0
Entrez Gene ID: (Human) 84632, (Rat) 292130, (Mouse) 226250
inflammatory response
regulation of mitotic cell cycle
regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of protein tyrosine kinase activity
positive regulation of signal transduction
regulation of signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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