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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
FIGURE: 1 / 4
AGO1 Antibody (711755) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), NTERA-2 cl.D1 (Lane 2), HEL 92.1.7 (Lane 3), K-562 (Lane 4), MOLT4 (Lane 5), A-431 (Lane 6), THP-1 (Lane 7), MDA-MB-231 (Lane 8) and T-47D (Lane 9). The blots were probed with Anti-AGO1 Recombinant Rabbit Superclonal™ Antibody (Product # 711755, 1-2 µg/mL). A 97 kDa band corresponding to AGO1 was observed across most of the cell lines tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondar... View More
Product Details
711755
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Bovine
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Peptides corresponding to human AGO1 [1) aa17-aa35, 2) aa382-aa401]
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2692263
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Mouse
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
AGO1 is a member of the Argonaute family of proteins which play a role in RNA interference. The protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. AGO1 binds to miRNAs or siRNAs and represses the translation of mRNAs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: argonaute 1; Argonaute RISC catalytic component 1; Argonaute1; eIF-2C 1; eIF2C 1; Eukaryotic translation initiation factor 2C 1; eukaryotic translation initiation factor 2C, 1; Golgi Endoplasmic Reticulum protein 95 kDa; mAgo1; Piwi/Argonaute family protein meIF2C1; Protein argonaute-1; Putative RNA-binding protein Q99
Gene Aliases: AGO1; EIF2C; EIF2C1; GERP95; hAgo1; Q99
UniProt ID: (Human) Q9UL18, (Mouse) Q8CJG1
Entrez Gene ID: (Human) 26523, (Mouse) 236511
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core binding
core promoter binding
RNA binding
double-stranded RNA binding
single-stranded RNA binding
endoribonuclease activity
protein binding
miRNA binding
poly(A) RNA binding
nucleic acid binding
transcription regulatory region DNA binding
pre-miRNA binding
translation initiation factor
nuclear-transcribed mRNA catabolic process
transcription, DNA-templated
Wnt signaling pathway, calcium modulating pathway
RNA secondary structure unwinding
miRNA metabolic process
gene silencing by RNA
pre-miRNA processing
posttranscriptional gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
miRNA loading onto RISC involved in gene silencing by miRNA
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol-mediated signaling
mRNA cleavage involved in gene silencing by siRNA
regulation of transcription, DNA-templated
regulation of translation
positive regulation of gene expression
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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