Invitrogen

AHR Monoclonal Antibody (4MEJJ), Alexa Fluor™ 488, eBioscience™

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AHR Antibody (53-5925-82) in Flow

Splenocytes from C57BL/6 mice were polarized under Th17 conditions for 3 days, stained with Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), fixed and permeabilized with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00), and then intracellularly stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and 0.25 µg of Rat IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (left) or 0.25 µg of Anti-Mouse AHR Alexa Fluor® 488 (right). Total viable cells were used for analysis.

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产品信息

53-5925-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

4MEJJ

抗原

E. coli derived, His-tagged AHR (aa 443-805)

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偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2574425

产品详细信息

Description: This 4MEJJ monoclonal antibody recognizes mouse aryl hydrocarbon receptor (AHR). The AHR is a ligand-activated transcription factor that mediates the toxic effects of a diverse group of environmental contaminants, most notably aryl hydrocarbons such as polychlorinated biphenyls (PCB) and tetrachlorodibenzo-p-dioxin (TCDD). The AHR has also been shown to bind to a number of naturally occurring compounds found in fruits and vegetables as well as compounds generated through normal cellular metabolism. AHR is localized in the cytoplasm in a complex that includes HSP90, p23, and XAP2/AIP/ARA9. Upon ligand-binding, AHR translocates to the nucleus and binds with aryl hydrocarbon receptor nuclear translocator (ARNT), and this complex binds to the consensus DNA sequence, GCGTG, found in the promoter/enhancer regions of many genes such as CYP1A1. The AHR is expressed in many cell types, with highest expression levels found in liver. The AHR has been shown to play a role in the regulation/differentiation of Treg and Th17 cells.

The 4MEJJ monoclonal antibody has been found to recognize the b-1, b-2 and d alleles of mouse AHR but does not crossreact with human AHR.

Applications Reported: This 4MEJJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 4MEJJ antibody has been tested by intracellular staining and flow cytometric analysis of TH17 polarized mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

The neutrophil antimicrobial peptide cathelicidin promotes Th17 differentiation.

Nature communications

Minns D,Smith KJ,Alessandrini V,Hardisty G,Melrose L,Jackson-Jones L,MacDonald AS,Davidson DJ,Gwyer Findlay E

Published figure using AHR monoclonal antibody (Product # 53-5925-82) in Flow Cytometry

Wed Feb 24 00:00:00 EST 2021

Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine.

Nature communications

Campesato LF,Budhu S,Tchaicha J,Weng CH,Gigoux M,Cohen IJ,Redmond D,Mangarin L,Pourpe S,Liu C,Zappasodi R,Zamarin D,Cavanaugh J,Castro AC,Manfredi MG,McGovern K,Merghoub T,Wolchok JD

Published figure using AHR monoclonal antibody (Product # 53-5925-82) in Flow Cytometry

Tue Aug 11 00:00:00 EDT 2020

Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence.

PLoS pathogens

Clement M,Marsden M,Stacey MA,Abdul-Karim J,Gimeno Brias S,Costa Bento D,Scurr MJ,Ghazal P,Weaver CT,Carlesso G,Clare S,Jones SA,Godkin A,Jones GW,Humphreys IR

53-5925 was used in Flow cytometry/Cell sorting to reveal that cytokine signalling events during initial infection profoundly influence virus chronicity.