Invitrogen

AHR Recombinant Rabbit Monoclonal Antibody (JM34-10)

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

查看其他39个AHR抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (5)
高级验证 (1)

AHR Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

AHR Antibody (MA5-32576) in ICC/IF

Immunocytochemical analysis of AHR in NIH/3T3 cells using a AHR Monoclonal antibody (Product # MA5-32576) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Image Gallery

AHR Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inAHR KO cell line compared to control cell line using AHR Recombinant Rabbit Monoclonal Antibody (JM34-10) (Product # MA5-32576). {KO}

产品信息

MA5-32576

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JM34-10

抗原

Synthetic peptide within Human Aryl hydrocarbon Receptor aa 735-775

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809853

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。