Invitrogen

AID Monoclonal Antibody (mAID-2), eBioscience™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

17个已发表图片

19 篇参考文献

查看其他13个AID抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

AID Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 20

AID Antibody (14-5959-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using low pH antigen retrieval and 20 µg/mL of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (top) or 20 µg/mL Anti-Human/Mouse AID (bottom) followed by Anti-Rat Biotin and DAB visualization.Nuclei are counterstained with hematoxylin.

AID Antibody in Immunohistochemistry (IHC)

AID Antibody in Western Blot, Flow Cytometry (WB, Flow)

AID Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

AID Antibody — Fig 1 TGF-beta1 upregulates APOBEC3 expression and suppresses HBV replication in Huh7 cells. Six hours after transfection of pPB, Huh7 cells were treated with TGF-beta1 for 3 days, and HBV replication was evaluated. (A) qRT-PCR shows dose-dependent reduction of HBV transcripts by TGF-beta1. (B) NC-DNA levels in secreted virions were also measured using qPCR. (C) Nucleocapsid NC-DNA and core protein levels in crude cytoplasmic extracts were assessed using NAGE assays. GAPDH protein levels in the same crude extracts were determined using western blotting. (D) Huh7 cells were treated with 150 ng/mL IL-4 or 10 ng/ml TGF-beta1 for 3 days. Levels of HBV RNA and GAPDH mRNA were determined by Northern blot. Control: non-stimulated Huh7 cells. (E) Relative expression levels of APOBEC deaminases in non-stimulated Huh7 cells; Relative expression levels were determined using qPCR with cDNA from non-stimulated Huh7 cells and standard curves of control APOBEC deaminase DNA. Relative copy numbers of A3B were defined as one. (F) Induction of APOBEC deaminase expression in TGF-beta1-treated Huh7 cells was estimated using qRT-PCR. Fold induction of APOBEC deaminases is shown in the top (10 ng/mL TGF-beta1 for 24 or 48 h) and bottom (10 or 20 ng/mL TGF-beta1 for 24 h) panels. (G) Huh7 cells were treated with indicated concentrations of TGF-beta1 for 3 days. AID protein was immunoprecipitated using an anti-AID antibody (or an isotype control IgG, most right) and immunoprecipitated AID protein wa {TM}

产品信息

14-5959-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

20 µg/mL

查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

20 µg/mL

免疫组化（冰冻） (IHC (F))

20 µg/mL

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀实验 (ChIP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

mAID-2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10669583

产品详细信息

Description: The monoclonal mAID-2 recognizes human and mouse AID (gene AICDA) also known as Activation-Induced Cytidine Deaminase. AID is a 24 kDa RNA-editing enzyme that converts cytosine into uracil. It plays a critical role during B cell development, specifically in somatic hypermutation (SHM) and immunoglobulin class switch recombination (CSR). Changes in levels of AID expression typically present with disease. For example, mutations in AID are found in Hyper-IgM Syndrome (type 2), a disease form without opportunistic infections, while overexpression correlates with poor prognosis in chronic lymphocytic leukemia/small lymphocytic lymphoma.

AID is expressed mainly by activated mature B cells such as mature germinal centre (GC) B cells but also in intermediate GC cells (defined as IgD+CD38-CD23-CD71+). In addition to hematopoietic cells, AID is expressed in primary human hepatocellular carcinomas, helicobacter infected gastric epithelial cells, oocytes and embryonic stem cells although it role in these cells is not well understood. Because AID contains an NLS (nuclear localization signal), expression can be found both in the nucleus as well as in the cytoplasm.

Applications Reported: This mAID-2 antibody has been reported for use in immunoblotting (WB), immunohistologic staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This mAID-2 antibody has been tested by western blot and/or immunohistochemistry on FFPE sections using either low or high pH antigen retrieval. This can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ATR kinase activity promotes antibody class switch recombination in B cells through cell cycle regulation without suppressing DSB resection and microhomology usage.

Journal of leukocyte biology

Sun X,Liu M,Bai J,Xu J,Zhu C,Dong J,Chen C

Published figure using AID monoclonal antibody (Product # 14-5959-82) in Western Blot

Wed Dec 01 00:00:00 EST 2021

The 3'-flap endonuclease XPF-ERCC1 promotes alternative end joining and chromosomal translocation during B cell class switching.

Cell reports

Bai W,Zhu G,Xu J,Chen P,Meng F,Xue H,Chen C,Dong J

14-5959-82 was used in Western Blot to reveal the role of the flap endonuclease, XPF-ERCC1, in alternative end joining and oncogenic translocation in mouse B cells.

Tue Sep 28 00:00:00 EDT 2021

Activation-induced cytidine deaminase is a possible regulator of cross-talk between oocytes and granulosa cells through GDF-9 and SCF feedback system.

Scientific reports

Iizuka T,Wakae K,Ono M,Suzuki T,Mizumoto Y,Kitamura K,Horike SI,Muramatsu M,Fujiwara H

14-5959-82 was used in Immunohistochemistry to study activation-induced cytidine deaminase expression in murine ovaries.

Mon Feb 15 00:00:00 EST 2021

Immunoglobulin Class Switch Recombination Is Initiated by Rare Cytosine Deamination Events at Switch Regions.

Molecular and cellular biology

Kim A,Han L,Yu K

14-5959 was used in Western Blotting to examine AID footprints across the entire length of an engineered switch region in cells ablated for uracil repair.

Wed Jul 29 00:00:00 EDT 2020

Interplay between UNG and AID governs intratumoral heterogeneity in mature B cell lymphoma.

PLoS genetics

Delgado P,Álvarez-Prado ÁF,Marina-Zárate E,Sernandez IV,Mur SM,de la Barrera J,Sanchez-Cabo F,Cañamero M,de Molina A,Belver L,de Yébenes VG,Ramiro AR

14-5959-82 was used in Western Blot to show using a conditional knock-in strategy that AID supra-activity alone is not sufficient to generate B cell transformation.

Tue Dec 01 00:00:00 EST 2020

Interplay between UNG and AID governs intratumoral heterogeneity in mature B cell lymphoma.

PLoS genetics

Delgado P,Álvarez-Prado ÁF,Marina-Zárate E,Sernandez IV,Mur SM,de la Barrera J,Sanchez-Cabo F,Cañamero M,de Molina A,Belver L,de Yébenes VG,Ramiro AR

14-5959-82 was used in Western Blot to show using a conditional knock-in strategy that AID supra-activity alone is not sufficient to generate B cell transformation.

Tue Dec 01 00:00:00 EST 2020

Liver Is a Generative Site for the B Cell Response to Ehrlichia muris.

Immunity

Trivedi N,Weisel F,Smita S,Joachim S,Kader M,Radhakrishnan A,Clouser C,Rosenfeld AM,Chikina M,Vigneault F,Hershberg U,Ismail N,Shlomchik MJ

14-5959-82 was used in Flow Cytometry to investigate the nature of liver B cell and spleen response to Ehrlichia muris.

Tue Dec 17 00:00:00 EST 2019

A licensing step links AID to transcription elongation for mutagenesis in B cells.

Nature communications

Methot SP,Litzler LC,Subramani PG,Eranki AK,Fifield H,Patenaude AM,Gilmore JC,Santiago GE,Bagci H,Côté JF,Larijani M,Verdun RE,Di Noia JM

14-5959 was used in Immunocytochemistry-immunoflourescence to study the mechanisms underlying productive deamination compared with non-productive activation-induced deaminase targeting.

Wed Mar 28 00:00:00 EDT 2018

Phosphatidylinositol 3-kinase δ blockade increases genomic instability in B cells.

Nature

Compagno M,Wang Q,Pighi C,Cheong TC,Meng FL,Poggio T,Yeap LS,Karaca E,Blasco RB,Langellotto F,Ambrogio C,Voena C,Wiestner A,Kasar SN,Brown JR,Sun J,Wu CJ,Gostissa M,Alt FW,Chiarle R

Published figure using AID monoclonal antibody (Product # 14-5959-82) in Western Blot

Thu Feb 23 00:00:00 EST 2017

Phosphatidylinositol 3-kinase δ blockade increases genomic instability in B cells.

Nature

Compagno M,Wang Q,Pighi C,Cheong TC,Meng FL,Poggio T,Yeap LS,Karaca E,Blasco RB,Langellotto F,Ambrogio C,Voena C,Wiestner A,Kasar SN,Brown JR,Sun J,Wu CJ,Gostissa M,Alt FW,Chiarle R

Published figure using AID monoclonal antibody (Product # 14-5959-82) in Western Blot

Thu Feb 23 00:00:00 EST 2017

The cell cycle restricts activation-induced cytidine deaminase activity to early G1.

The Journal of experimental medicine

Wang Q,Kieffer-Kwon KR,Oliveira TY,Mayer CT,Yao K,Pai J,Cao Z,Dose M,Casellas R,Jankovic M,Nussenzweig MC,Robbiani DF

14-5959 was used in Western Blotting to show that activation-induced cytidine deaminase (AID) deaminates cytosine after cytokinesis in early G1, and that processing of these lesions continues throughout the G1 phase of the cell cycle.

Sun Jan 01 00:00:00 EST 2017

CCCTC-Binding Factor Locks Premature IgH Germline Transcription and Restrains Class Switch Recombination.

Frontiers in immunology

Marina-Zárate E,Pérez-García A,Ramiro AR

Mon Jan 31 00:00:00 EST 2022

Epstein-Barr virus nuclear protein EBNA3C directly induces expression of AID and somatic mutations in B cells.

The Journal of experimental medicine

Kalchschmidt JS,Bashford-Rogers R,Paschos K,Gillman AC,Styles CT,Kellam P,Allday MJ

14595982 was used in western blot to demonstrate induction of functional AID by EBNA3C

Mon May 30 00:00:00 EDT 2016

Epstein-Barr virus nuclear protein EBNA3C directly induces expression of AID and somatic mutations in B cells.

The Journal of experimental medicine

Kalchschmidt JS,Bashford-Rogers R,Paschos K,Gillman AC,Styles CT,Kellam P,Allday MJ

14595982 was used in western blot to demonstrate induction of functional AID by EBNA3C

Mon May 30 00:00:00 EDT 2016

TGF-β suppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex.

PLoS pathogens

Liang G,Liu G,Kitamura K,Wang Z,Chowdhury S,Monjurul AM,Wakae K,Koura M,Shimadu M,Kinoshita K,Muramatsu M

14-5959 was used in Western Blotting to show that reduction of HBV transcripts by TGF-β is dependent on AID expression and can inhibit viral replication.

Wed Apr 01 00:00:00 EDT 2015

TGF-β suppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex.

PLoS pathogens

Liang G,Liu G,Kitamura K,Wang Z,Chowdhury S,Monjurul AM,Wakae K,Koura M,Shimadu M,Kinoshita K,Muramatsu M

14-5959 was used in Western Blotting to show that reduction of HBV transcripts by TGF-β is dependent on AID expression and can inhibit viral replication.

Wed Apr 01 00:00:00 EDT 2015

HSP90 inhibitors decrease AID levels and activity in mice and in human cells.

European journal of immunology

Montamat-Sicotte D,Litzler LC,Abreu C,Safavi S,Zahn A,Orthwein A,Müschen M,Oppezzo P,Muñoz DP,Di Noia JM

Published figure using AID monoclonal antibody (Product # 14-5959-82) in Western Blot

Sat Aug 01 00:00:00 EDT 2015

HSP90 inhibitors decrease AID levels and activity in mice and in human cells.

European journal of immunology

Montamat-Sicotte D,Litzler LC,Abreu C,Safavi S,Zahn A,Orthwein A,Müschen M,Oppezzo P,Muñoz DP,Di Noia JM

Published figure using AID monoclonal antibody (Product # 14-5959-82) in Western Blot

Sat Aug 01 00:00:00 EDT 2015

HSP90 inhibitors decrease AID levels and activity in mice and in human cells.

European journal of immunology

Montamat-Sicotte D,Litzler LC,Abreu C,Safavi S,Zahn A,Orthwein A,Müschen M,Oppezzo P,Muñoz DP,Di Noia JM

Published figure using AID monoclonal antibody (Product # 14-5959-82) in Western Blot

Sat Aug 01 00:00:00 EDT 2015

HSP90 inhibitors decrease AID levels and activity in mice and in human cells.

European journal of immunology

Montamat-Sicotte D,Litzler LC,Abreu C,Safavi S,Zahn A,Orthwein A,Müschen M,Oppezzo P,Muñoz DP,Di Noia JM

Published figure using AID monoclonal antibody (Product # 14-5959-82) in Western Blot

Sat Aug 01 00:00:00 EDT 2015

The transcription factor Foxp1 is a critical negative regulator of the differentiation of follicular helper T cells.

Nature immunology

Wang H,Geng J,Wen X,Bi E,Kossenkov AV,Wolf AI,Tas J,Choi YS,Takata H,Day TJ,Chang LY,Sprout SL,Becker EK,Willen J,Tian L,Wang X,Xiao C,Jiang P,Crotty S,Victora GD,Showe LC,Tucker HO,Erikson J,Hu H

14-5959 was used in Immunohistochemistry to investigate the role of Foxp1 in CD4(+) cells, showing that it is a critical negative regulator of the differentiation of follicular helper T cells.

Tue Jul 01 00:00:00 EDT 2014

Generation and characterization of induced pluripotent stem cells from Aid-deficient mice.

PloS one

Shimamoto R,Amano N,Ichisaka T,Watanabe A,Yamanaka S,Okita K

14-5959 was used in Western Blotting to investigate the underlying mechanism of DNA demethylation in generation of induced pluripotent stem cells.

Mon Jun 29 00:00:00 EDT 2015

BCL6 positively regulates AID and germinal center gene expression via repression of miR-155.

The Journal of experimental medicine

Basso K,Schneider C,Shen Q,Holmes AB,Setty M,Leslie C,Dalla-Favera R

14-5959 was used in Immunofluorescence to investigate the potential role of BCL6 in regulating gene expression via microRNAs.

Mon Dec 17 00:00:00 EST 2012

生物信息学

蛋白别名: activation induced deaminase; Activation-induced cytidine deaminase; AID; Cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos; Single-stranded DNA cytosine deaminase

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基因别名: AICDA; AID; ARP2; CDA2; HEL-S-284; HIGM2

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UniProt ID: (Human) Q9GZX7, (Mouse) Q9WVE0

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Entrez Gene ID: (Human) 57379, (Mouse) 11628

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功能

cytidine deaminase activity protein binding zinc ion binding ubiquitin protein ligase binding catalytic activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines metal ion binding RNA processing factor

参与通路

mRNA processing cytidine deamination somatic diversification of immunoglobulins somatic hypermutation of immunoglobulin genes B cell differentiation isotype switching cellular response to lipopolysaccharide DNA demethylation negative regulation of methylation-dependent chromatin silencing

AID Monoclonal Antibody (mAID-2), eBioscience™

AID Antibody (14-5959-82) in IHC (P)

产品信息

14-5959-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

AID Monoclonal Antibody (mAID-2), eBioscience™

AID Antibody (14-5959-82) in IHC (P)

产品信息

14-5959-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)