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Application
FIGURE: 1 / 1
ALOX12 Antibody (PA5-77858) in WB
Western Blot using ALOX12 Polyclonal Antibody (Product # PA5-77858). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALOX12 Polyclonal Antibody (Product # PA5-77858) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-77858
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human ALOX12. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
2.96 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735446
Product Specific Information
Positive Control: A431
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Oxygenase and 14,15-leukotriene A4 synthase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12(S)-lipoxygenase; 12LOX; 12S LOX; 12S-lipoxygenase; 12S-LOX; Arachidonate (12S)-lipoxygenase; Arachidonate (15S)-lipoxygenase; arachidonate 12-lipoxygenase; Linoleate (13S)-lipoxygenase; Lipoxin synthase 12-LO; platelet 12-LOX; platelet-type 12-lipoxygenase; Platelet-type lipoxygenase 12; Polyunsaturated fatty acid lipoxygenase ALOX12
Gene Aliases: 12-LOX; 12LO; 12S-LOX; ALOX12; LOG12
UniProt ID: (Human) P18054
Entrez Gene ID: (Human) 239
arachidonate 12-lipoxygenase activity
iron ion binding
protein binding
linoleate 13S-lipoxygenase activity
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
hepoxilin-epoxide hydrolase activity
hepoxilin A3 synthase activity
oxygenase
movement of cell or subcellular component
aging
positive regulation of cell proliferation
positive regulation of endothelial cell migration
positive regulation of gene expression
negative regulation of muscle cell apoptotic process
arachidonic acid metabolic process
lipoxygenase pathway
fatty acid oxidation
positive regulation of cell growth
positive regulation of cell migration
superoxide anion generation
negative regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
linoleic acid metabolic process
positive regulation of endothelial cell differentiation
positive regulation of angiogenesis
positive regulation of cell adhesion
positive regulation of vasodilation
positive regulation of smooth muscle cell proliferation
hepoxilin metabolic process
hepoxilin biosynthetic process
positive regulation of mitochondrial depolarization
establishment of skin barrier
cellular response to lipid
reactive oxygen species metabolic process
negative regulation of platelet aggregation
leukotriene A4 metabolic process
lipoxin metabolic process
lipoxin A4 biosynthetic process
lipoxin B4 biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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