Invitrogen

ALPK2 Polyclonal Antibody

查看其他7个ALPK2抗体

抗体检测数据 (6)

ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ALPK2 Antibody (PA5-104538) in IHC (P)

Immunohistochemistry analysis of ALPK2 in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with ALPK2 polyclonal antibody (Product # PA5-104538) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALPK2 Antibody in Western Blot (WB)

ALPK2 Antibody in Western Blot (WB)

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ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ALPK2 in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with ALPK2 polyclonal antibody (Product # PA5-104538) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ALPK2 in human brain tissues sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ALPK2 polyclonal antibody (Product # PA5-104538) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ALPK2 in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with ALPK2 polyclonal antibody (Product # PA5-104538) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

ALPK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ALPK2 in rat brain tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ALPK2 polyclonal antibody (Product # PA5-104538) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ALPK2 Antibody in Western Blot (WB) — Western blot analysis of ALPK2 in mouse brain tissue lysate (left lane: treated with blocking peptide). Samples were incubated with ALPK2 polyclonal antibody (Product # PA5-104538).

ALPK2 Antibody in Western Blot (WB) — Western blot analysis of ALPK2 in mouse brain lysate. Samples were incubated with ALPK2 polyclonal antibody (Product # PA5-104538).

产品信息

PA5-104538

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human ALPK2(Accession Q86TB3), corresponding to amino acid residues L1742-I1792.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2853838

产品详细信息

Antibody detects endogenous levels of total ALPK2.

靶标信息

Protein kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation (PubMed:10021370). Regulates cardiac development and cardiomyocyte differentiation by negatively regulating Wnt/beta-catenin signaling (PubMed:29888752). [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: alpha-kinase 2; Alpha-protein kinase 2; alpk2; heart alpha-kinase; Heart alpha-protein kinase; RNF18

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基因别名: ALPK2; Gm549; HAK; RGD1564431

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UniProt ID: (Human) Q86TB3, (Mouse) Q91ZB0

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Entrez Gene ID: (Human) 115701, (Mouse) 225638, (Rat) 498875

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protein serine/threonine kinase activity ATP binding molecular_function transmembrane receptor protein serine/threonine kinase activity 3-phosphoinositide-dependent protein kinase activity DNA-dependent protein kinase activity AMP-activated protein kinase activity cyclic nucleotide-dependent protein kinase activity eukaryotic translation initiation factor 2alpha kinase activity calcium-dependent protein kinase C activity receptor signaling protein serine/threonine kinase activity NF-kappaB-inducing kinase activity JUN kinase activity JUN kinase kinase kinase activity MAP kinase kinase activity MAP kinase kinase kinase activity ribosomal protein S6 kinase activity MAP kinase kinase kinase kinase activity JUN kinase kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SAP kinase activity transmembrane receptor protein kinase activity cyclin-dependent protein kinase activating kinase activity GTP-dependent protein kinase activity non-receptor serine/threonine protein kinase

参与通路

protein phosphorylation biological_process phosphorylation