Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 7
AMFR Antibody (PA5-78768) in ICC/IF
Immunocytochemistry analysis of AMFR using anti-AMFR antibody (Product # PA5-78768) . AMFR was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-AMFR antibody (Product # PA5-78768) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was countersta... View More
产品信息
PA5-78768
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human AMFR recombinant protein (Position: E553-S643).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2745884
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: AMF receptor; Autocrine motility factor receptor; autocrine motility factor receptor, E3 ubiquitin protein ligase; E3 ubiquitin-protein ligase AMFR; gp78; RING finger protein 45; RING-type E3 ubiquitin transferase AMFR
基因别名: AMFR; GP78; RNF45
UniProt ID: (Human) Q9UKV5, (Mouse) Q9R049
Entrez Gene ID: (Human) 267, (Rat) 361367, (Mouse) 23802
ubiquitin-protein transferase activity
receptor activity
protein binding
zinc ion binding
ligase activity
protein binding, bridging
ubiquitin-ubiquitin ligase activity
chaperone binding
ubiquitin protein ligase activity
ubiquitin protein ligase activity involved in ERAD pathway
BAT3 complex binding
ubiquitin-specific protease binding
molecular_function
nucleotide binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
protein polyubiquitination
ubiquitin-dependent protein catabolic process
movement of cell or subcellular component
signal transduction
aging
learning or memory
ER-associated ubiquitin-dependent protein catabolic process
endoplasmic reticulum unfolded protein response
positive regulation of protein binding
ERAD pathway
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
protein oligomerization
protein autoubiquitination
protein K48-linked ubiquitination
cellular process
