Invitrogen

AP2 alpha Polyclonal Antibody

查看其他14个AP2 alpha抗体

抗体检测数据 (6)

AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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AP2 alpha Antibody (PA5-105277) in IHC (P)

Immunohistochemistry analysis of AP2 alpha in human gastric cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with AP2 alpha polyclonal antibody (Product # PA5-105277) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AP2 alpha Antibody in Western Blot (WB)

AP2 alpha Antibody in Western Blot (WB)

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AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of AP2 alpha in human gastric cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with AP2 alpha polyclonal antibody (Product # PA5-105277) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of AP2 alpha in human esophageal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with AP2 alpha polyclonal antibody (Product # PA5-105277) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of AP2 alpha in mouse spleen tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with AP2 alpha polyclonal antibody (Product # PA5-105277) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

AP2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of AP2 alpha in mouse liver tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with AP2 alpha polyclonal antibody (Product # PA5-105277) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

AP2 alpha Antibody in Western Blot (WB) — Western blot analysis of AP2 alpha in COLO205 cell lysate (left lane: treated with blocking peptide). Samples were incubated with AP2 alpha polyclonal antibody (Product # PA5-105277).

AP2 alpha Antibody in Western Blot (WB) — Western blot analysis of AP2 alpha in COLO205 cell lysate. Samples were incubated with AP2 alpha polyclonal antibody (Product # PA5-105277).

产品信息

PA5-105277

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human TFAP2A(Accession P05549), corresponding to amino acid residues K411-K437.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2853879

产品详细信息

Antibody detects endogenous levels of total AP2 alpha.

靶标信息

TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: activating enhancer-binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 transcription factor; AP2-alpha; FLJ51761; OTTHUMP00000214240; OTTHUMP00000214243; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha

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基因别名: AP-2; Ap-2 (a); AP-2alpha; Ap2; AP2alpha; AP2TF; BOFS; Tcfap2a; TFAP2; TFAP2A

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UniProt ID: (Human) P05549, (Mouse) P34056

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Entrez Gene ID: (Human) 7020, (Mouse) 21418, (Rat) 306862

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transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription coactivator activity protein binding transcription factor binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding protein dimerization activity RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription coactivator activity RNA polymerase II transcription corepressor activity transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding general transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter kidney development optic vesicle morphogenesis optic cup structural organization transcription from RNA polymerase II promoter sensory perception of sound negative regulation of cell proliferation response to water deprivation positive regulation of gene expression retina layer formation negative regulation of transcription by competitive promoter binding Schwann cell development trigeminal nerve development oculomotor nerve formation positive regulation of bone mineralization response to lipopolysaccharide embryonic forelimb morphogenesis inner ear morphogenesis negative regulation of apoptotic process positive regulation of neuron apoptotic process regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic cranial skeleton morphogenesis palate development bone morphogenesis eyelid development in camera-type eye positive regulation of tooth mineralization cellular response to iron ion negative regulation of reactive oxygen species metabolic process neural tube closure lens morphogenesis in camera-type eye outflow tract morphogenesis keratinocyte development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter embryonic pattern specification embryonic body morphogenesis neural crest cell development anterior neuropore closure forebrain neuron development positive regulation of cell migration forelimb morphogenesis negative regulation of epidermal growth factor receptor signaling pathway negative regulation of neuron apoptotic process skin development regulation of neuron differentiation sympathetic nervous system development embryonic camera-type eye morphogenesis skeletal system morphogenesis epidermis morphogenesis lens induction in camera-type eye face morphogenesis cornea development in camera-type eye basement membrane organization response to organic substance regulation of cell proliferation metanephric nephron development