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APC5 Antibody (PA5-96581) in IHC (P)
Immunohistochemistry analysis of APC5 in paraffin-embedded human kidney. Samples were incubated with APC5 Polyclonal antibody (Product # PA5-96581) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
产品信息
PA5-96581
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_0573212)
偶联物
Unconjugated
形式
Liquid
浓度
0.21 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2808383
产品详细信息
Immunogen sequence: MASVHESLYF NPMMTNGVVH ANVFGIKDWV TPYKIAVLVL LNEMSRTGEG AVSLMERRRL NQLLLPLLQG PDITLSKLYK LIEESCPQLA NSVQIRIKLM AEGELKDMEQ FFDDLSDSFS GTEPEVHKTS VVGLFLRHMI LAYSKLSFSQ VFKLYTALQQ YFQNGEKKTV EDADMELTSR DEGERKMEKE ELDVSVREEE VSCSGPLSQK QAEFFLSQQA SLLKNDETKA LTPASLQKEL NNLLKFNPDF; Positive Samples: SW620, MCF-7, HL-60, 22Rv1, A-549, Mouse spleen
靶标信息
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome, a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein, compared to isoform a.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Anaphase-promoting complex subunit 5; APC5; Cyclosome subunit 5
基因别名: 2510006G12Rik; AA408751; AA536819; AA986414; ANAPC5; Anpc5; APC5; MNCb-2778
UniProt ID: (Human) Q9UJX4, (Rat) A1L1K3, (Mouse) Q8BTZ4
Entrez Gene ID: (Human) 51433, (Rat) 288671, (Mouse) 59008
mitotic nuclear division
cell division
protein K11-linked ubiquitination
anaphase-promoting complex-dependent catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
cell cycle
