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Invitrogen
APEX2 Polyclonal Antibody
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FIGURE: 1 / 2
APEX2 Antibody (PA5-78791) in WB
Western blot analysis of APEX2 in, Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate, Lane 3: HEK293 whole cell lysate, Lane 4: COS-7 whole cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at ... View More
Product Details
PA5-78791
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human APEX2 recombinant protein (Position: L102-A210).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue, HEK293 whole cell, COS-7 whole cell. Flow: A549 cell.
Target Information
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair (BER).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AP endonuclease 2; AP endonuclease XTH2; APEX nuclease (apurinic/apyrimidinic endonuclease) 2; APEX nuclease 2; APEX nuclease-like 2; Apurinic-apyrimidinic endonuclease 2; apurinic/apyrimidinic endonuclease-like 2; DNA-(apurinic or apyrimidinic site) endonuclease 2; DNA-(apurinic or apyrimidinic site) lyase 2; RP5-884M20.3; zinc finger, GRF-type containing 2
Gene Aliases: APE2; APEX2; APEXL2; C430040P13Rik; XTH2; ZGRF2
UniProt ID: (Human) Q9UBZ4, (Mouse) Q68G58
Entrez Gene ID: (Human) 27301, (Rat) 317628, (Mouse) 77622
DNA binding
DNA-(apurinic or apyrimidinic site) lyase activity
zinc ion binding
double-stranded DNA 3'-5' exodeoxyribonuclease activity
nuclease activity
endonuclease activity
exonuclease activity
hydrolase activity
lyase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
metal ion binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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