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APLF Antibody (PA5-114861) in ICC/IF
Immunocytochemistry analysis of APLF in A549 cells (H2O2 treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with APLF polyclonal antibody (Product # PA5-114861) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-114861
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human APLF(Accession Q8IW19), corresponding to amino acid residues L92-G142.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2899497
产品详细信息
Antibody detects endogenous levels of total APLF.
靶标信息
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks.[supplied by OMIM, Mar 2008].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Aprataxin and PNK-like factor; aprataxin- and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; XRCC1-interacting protein 1; zinc finger, CX5CX6HX5H motif containing 1
基因别名: 2010301N04Rik; AI452191; APFL; APLF; C2orf13; PALF; RGD1565557; XIP1; ZCCHH1
UniProt ID: (Human) Q8IW19, (Mouse) Q9D842
Entrez Gene ID: (Human) 200558, (Rat) 500247, (Mouse) 72103
