Invitrogen

APOE Recombinant Rabbit Monoclonal Antibody (16H22L18)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

9个已发表图片

11 篇参考文献

查看其他28个APOE抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

APOE Antibody in Immunocytochemistry (ICC/IF)

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APOE Antibody (701241) in ICC/IF

Immunofluorescence analysis of APOE was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with APOE Recombinant Rabbit Monoclonal Antibody (16H22L18) (Product # 701241) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, ... View More

APOE Antibody in Immunohistochemistry (Frozen), Proximity Ligation Assay (PLA) (IHC (F), PLA)

APOE Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

APOE Antibody in Immunohistochemistry, Proximity Ligation Assay (PLA) (IHC, PLA)

Product Image Gallery

APOE Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of APOE was observed in Hep G2, CaCo-2 as compared to A-431, Daudi using Anti-APOE Recombinant Rabbit Monoclonal Antibody (16H22L18) (Product # 701241) in Western Blot. {RE}

产品信息

701241

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1-3 µg/mL

查看1篇已发表文章 1篇已发表文章

in situ PLA (PLA)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

16H22L18

抗原

Peptide corresponding to amino acids 240-251 of human ApoE

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532438

产品详细信息

This antibody is predicted to react with non-human primate and mouse based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Enhanced liver X receptor signalling reduces brain injury and promotes tissue regeneration following experimental intracerebral haemorrhage: roles of microglia/macrophages.

Stroke and vascular neurology

Zhang R,Dong Y,Liu Y,Moezzi D,Ghorbani S,Mirzaei R,Lozinski BM,Dunn JF,Yong VW,Xue M

701241 was used in Immunohistochemistry (Frozen) to examine the benefits of enhanced Liver X receptor signaling in experimental intracerebral hemorrhage.

Fri Dec 29 00:00:00 EST 2023

Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Aging cell

Zhang Z,Na H,Gan Q,Tao Q,Alekseyev Y,Hu J,Yan Z,Yang JB,Tian H,Zhu S,Li Q,Rajab IM,Blusztajn JK,Wolozin B,Emili A,Zhang X,Stein T,Potempa LA,Qiu WQ

Published figure using APOE recombinant monoclonal antibody (Product # 701241) in Immunohistochemistry

Mon Nov 01 00:00:00 EDT 2021

Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Aging cell

Zhang Z,Na H,Gan Q,Tao Q,Alekseyev Y,Hu J,Yan Z,Yang JB,Tian H,Zhu S,Li Q,Rajab IM,Blusztajn JK,Wolozin B,Emili A,Zhang X,Stein T,Potempa LA,Qiu WQ

Published figure using APOE recombinant monoclonal antibody (Product # 701241) in Immunohistochemistry

Mon Nov 01 00:00:00 EDT 2021

Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Aging cell

Zhang Z,Na H,Gan Q,Tao Q,Alekseyev Y,Hu J,Yan Z,Yang JB,Tian H,Zhu S,Li Q,Rajab IM,Blusztajn JK,Wolozin B,Emili A,Zhang X,Stein T,Potempa LA,Qiu WQ

Published figure using APOE recombinant monoclonal antibody (Product # 701241) in Immunohistochemistry

Mon Nov 01 00:00:00 EDT 2021

ApoE4 inhibition of VMAT2 in the locus coeruleus exacerbates Tau pathology in Alzheimer's disease.

Acta neuropathologica

Kang SS,Ahn EH,Liu X,Bryson M,Miller GW,Weinshenker D,Ye K

701241 was used in Western Blotting to show that ApoE4 selectively binds to the vesicular monoamine transporter 2 (VMAT2) and inhibits neurotransmitter uptake and that ApoE4 exacerbates Tau neurotoxicity by increasing VMAT2 vesicle leakage and facilitating AEP-mediated Tau proteolytic cleavage in the LC via DOPEGAL.

Thu Jul 01 00:00:00 EDT 2021

C/EBPβ is a key transcription factor for APOE and preferentially mediates ApoE4 expression in Alzheimer's disease.

Molecular psychiatry

Xia Y,Wang ZH,Zhang J,Liu X,Yu SP,Ye KX,Wang JZ,Ye K,Wang XC

701241 was used in Western Blotting to find that C/EBPβ selectively promotes more ApoE4 expression versus ApoE3 in human neurons, correlating with higher activation of C/EBPβ in human AD brains with ApoE4/4 compared to ApoE3/3.

Fri Oct 01 00:00:00 EDT 2021

Monocyte-derived alveolar macrophage apolipoprotein E participates in pulmonary fibrosis resolution.

JCI insight