Invitrogen

ARF1 Recombinant Rabbit Monoclonal Antibody (23GB3155)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他17个ARF1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

ARF1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

ARF1 Antibody (MA5-53372) in ICC/IF

Immunocytochemistry analysis of ARF1 in C2C12 cells. Nuclei were stained blue with DAPI; ARF1 recombinant monoclonal antibody (Product # MA5-53372) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Product Image Gallery

ARF1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with ADP ribosylation factor 1 shRNA and decrease in signal intensity was observed in Western Blotting using Anti-ARF1 Recombinant Rabbit Monoclonal Antibody (23GB3155) (Product # MA5-53372) at a dilution of 1:5,000. Secondary antibody used was a goat anti-rabbit (HRP) at a dilution of 1:20,000. {KD}

产品信息

MA5-53372

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫细胞化学 (ICC/IF)

1:1,000

流式细胞分析 (Flow)

1:2,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

23GB3155

抗原

A synthetic peptide of human ARF1 (70-108AA).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

靶标信息

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。