Invitrogen

ARF6 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

5个已发表图片

10 篇参考文献

查看其他10个ARF6抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

ARF6 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

ARF6 Antibody (PA1-093) in ICC/IF

Immunofluorescence analysis of ARF6 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ARF6 Polyclonal Antibody (Product # PA1-093) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000)... View More

ARF6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF6 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

ARF6 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MDA-MB-231 cells were transfected with ARF6 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-ARF6 Polyclonal Antibody (Product # PA1-093). {KD}

产品信息

PA1-093

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:100

免疫细胞化学 (ICC/IF)

1:20-1:100

查看3篇已发表文章 3篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Non-human primate

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

C-terminal human recombinant protein fragment

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539860

靶标信息

ARF6 (ADP-ribosylation factor 6) is a broadly expressed, differentially regulated GTPase that is present in cytoplasm and on both cell-surface and intracellular membranes and whose functions may include tissue-specific effects on vesicular trafficking during cellular differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SARS-CoV-2 pseudovirus enters the host cells through spike protein-CD147 in an Arf6-dependent manner.

Emerging microbes & infections

Zhou YQ,Wang K,Wang XY,Cui HY,Zhao Y,Zhu P,Chen ZN

PA1-093 was used in Immunocytochemistry to provide new insights into the CD147-Arf6 axis in mediating SARS-CoV-2 pseudovirus entry into the host cells, and further suggest that blockade of this pathway seems to be a feasible approach to prevent the SARS-CoV-2 infection clinically.

Thu Dec 01 00:00:00 EST 2022

SARS-CoV-2 pseudovirus enters the host cells through spike protein-CD147 in an Arf6-dependent manner.

Emerging microbes & infections

Zhou YQ,Wang K,Wang XY,Cui HY,Zhao Y,Zhu P,Chen ZN

Thu Dec 01 00:00:00 EST 2022

A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.

Nature communications

Sun P,Nie K,Zhu Y,Liu Y,Wu P,Liu Z,Du S,Fan H,Chen CH,Zhang R,Wang P,Cheng G

Published figure using ARF6 polyclonal antibody (Product # PA1-093) in Immunocytochemistry

Tue Jan 14 00:00:00 EST 2020

Arfgef1 haploinsufficiency in mice alters neuronal endosome composition and decreases membrane surface postsynaptic GABA_A receptors.

Neurobiology of disease

Teoh J,Subramanian N,Pero ME,Bartolini F,Amador A,Kanber A,Williams D,Petri S,Yang M,Allen AS,Beal J,Haut SR,Frankel WN

PA1-093 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that the altered endosome composition and decreased neuronal surface GABAAR results suggests a mechanism whereby impaired neuronal inhibition leads to seizure susceptibility.

Sat Feb 01 00:00:00 EST 2020

Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer.

Nature

Yao W,Rose JL,Wang W,Seth S,Jiang H,Taguchi A,Liu J,Yan L,Kapoor A,Hou P,Chen Z,Wang Q,Nezi L,Xu Z,Yao J,Hu B,Pettazzoni PF,Ho IL,Feng N,Ramamoorthy V,Jiang S,Deng P,Ma GJ,Den P,Tan Z,Zhang SX,Wang H,Wang YA,Deem AK,Fleming JB,Carugo A,Heffernan TP,Maitra A,Viale A,Ying H,Hanash S,DePinho RA,Draetta GF

PA1-093X was used in Western Blotting to investigate if a mechanistic link between KRAS* signaling and a targetable molecule driving nutrient salvage pathway in pancreatic ductal adenocarcinoma exists.

Mon Apr 01 00:00:00 EDT 2019

Heterozygous loss of function of IQSEC2/Iqsec2 leads to increased activated Arf6 and severe neurocognitive seizure phenotype in females.

Life science alliance

Jackson MR,Loring KE,Homan CC,Thai MH,Määttänen L,Arvio M,Jarvela I,Shaw M,Gardner A,Gecz J,Shoubridge C

PA1-093 was used in Western Blotting to confirm the loss of Iqsec2 mRNA expression and lack of Iqsec2 protein within the brain of both founder and progeny mice.