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ASAH1 Antibody (PA5-143869) in ICC/IF
Immunocytochemistry analysis of ASAH1 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in ASAH1 Polyclonal antibody (Product # PA5-143869) using a dilution of 2 μg/mL. DyLight®488 conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
产品信息
PA5-143869
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human ASAH1 recombinant protein (Position: H63-I393).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3075083
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
靶标信息
ASAH1 encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. The processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene have been linked to the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. The related pathways include Innate Immune System and Sphingolipid metabolism. Diseases associated with ASAH1 include Farber Lipogranulomatosis and Spinal Muscular Atrophy With Progressive Myoclonic Epilepsy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AC; ACDase; acid CDase; Acid ceramidase; Acylsphingosine deacylase; Glycosylceramide deacylase; N-acylethanolamine hydrolase ASAH1; N-acylsphingosine amidohydrolase; N-acylsphingosine amidohydrolase (acid ceramidase) 1; N-acylsphingosine amidohydrolase 1; PHP32; Putative 32 kDa heart protein
基因别名: 2310081N20Rik; AC; ACDase; ASAH; ASAH1; HSD-33; HSD33; PHP; PHP32; SMAPME
UniProt ID: (Human) Q13510, (Mouse) Q9WV54, (Rat) Q6P7S1
Entrez Gene ID: (Human) 427, (Mouse) 11886, (Rat) 84431
