Invitrogen

ASAH2 Polyclonal Antibody

产品信息

PA5-48033

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫沉淀 (IP)

25 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Sheep / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse ASAH2/N-acylsphingosine Amidohydrolase-2 Thr34-Thr756

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2577165

产品详细信息

In direct ELISAs, approximately 15% cross-reactivity with recombinant human ASAH2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.

仅用于科研。不用于诊断过程。未经明确授权不得转售。