Invitrogen

ASCT2 Polyclonal Antibody

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ASCT2 Antibody (PA5-50527) in WB

Western blot was performed using Anti-ASCT2 Polyclonal Antibody (Product # PA5-50527) and a 57 kDa and 80 kDa band corresponding to Neutral amino acid transporter B(0) was observed across cell lines. Whole cell extracts (60 µg lysate) of MCF7 (Lane 1), HT-29 (Lane 2), A549 (Lane 3), T-47D (Lane 4), Hep G2 (Lane 5), BeWo (Lane 6), THP-1 (Lane 7) shown in figure a, and 3T3-L1 (Lane 8), 3T3-L1 (Lane 9) shown in fig b, were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred o... View More

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产品信息

PA5-50527

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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流式细胞分析 (Flow)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region derived from internal residues of human Solute carrier family 1 (neutral amino acid transporter), member 5

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 40% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2635980

产品详细信息

The antibody detects endogenous levels of total SLC1A5 / ASCT2 protein.

靶标信息

SLC1A5 (Solute carrier family 1 (neutral amino acid transporter), member 5) is a member of the Na(+)-dependent amino acid transporter superfamily. It has a broad substrate specificity, a preference for zwitterionic amino acids, and a sodium-dependence. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated amino acids, anionic amino acids, and cationic amino acids. It acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Integrated Metabolic and Epigenomic Reprograming by H3K27M Mutations in Diffuse Intrinsic Pontine Gliomas.

Cancer cell

Chung C,Sweha SR,Pratt D,Tamrazi B,Panwalkar P,Banda A,Bayliss J,Hawes D,Yang F,Lee HJ,Shan M,Cieslik M,Qin T,Werner CK,Wahl DR,Lyssiotis CA,Bian Z,Shotwell JB,Yadav VN,Koschmann C,Chinnaiyan AM,Blüml S,Judkins AR,Venneti S

PA5-50527 was used in Western Blotting to show that H3K27M and IDH1 mutations are mutually exclusive and experimentally synthetic lethal.

Mon Sep 14 00:00:00 EDT 2020

CD38-NAD⁺Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response.

Cell metabolism

Chatterjee S,Daenthanasanmak A,Chakraborty P,Wyatt MW,Dhar P,Selvam SP,Fu J,Zhang J,Nguyen H,Kang I,Toth K,Al-Homrani M,Husain M,Beeson G,Ball L,Helke K,Husain S,Garrett-Mayer E,Hardiman G,Mehrotra M,Nishimura MI,Beeson CC,Bupp MG,Wu J,Ogretmen B,Paulos CM,Rathmell J,Yu XZ,Mehrotra S

PA5-50527 was used in Flow Cytometry to establish ex vivo culture conditions to generate hybrid Th1/17 cells, which persist long-term in vivo while maintaining effector function.

Tue Jan 09 00:00:00 EST 2018

生物信息学

蛋白别名: ASC-like Na(+)-dependent neutral amino acid transporter ASCT2; ATB(0); ATB0; Baboon M7 virus receptor; insulin-activated amino acid transporter; neutral amino acid transporter B; Neutral amino acid transporter B(0); RD114 virus receptor; RD114/simian type D retrovirus receptor; Sodium-dependent neutral amino acid transporter type 2; solute carrier family 1 (neutral amino acid transporter), member 5; Solute carrier family 1 member 5; solute carrier family 1, member 7

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基因别名: AAAT; ASCT2; ATBO; M7V1; M7VS1; R16; RDR; RDRC; SLC1A5; Slc1a7

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UniProt ID: (Human) Q15758

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Entrez Gene ID: (Human) 6510, (Mouse) 20514

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功能

virus receptor activity receptor activity protein binding amino acid transmembrane transporter activity neutral amino acid transmembrane transporter activity L-glutamine transmembrane transporter activity symporter activity L-serine transmembrane transporter activity sodium:dicarboxylate symporter activity primary active transporter

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