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Invitrogen
ATF Polyclonal Antibody
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FIGURE: 1 / 1
ATF Antibody (PA5-96076) in WB
Western blot analysis of ATF in extracts from normal (control) and ATF2 Rabbit pAb knockout (KO) 293T cells. Samples were incubated with ATF Polyclonal antibody (Product # PA5-96076) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-96076
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 110-190 of ATF2 (NP_0018712)
Conjugate
Form
Liquid
Concentration
2.18 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: DLSPLATPII RSKIEEPSVV ETTHQDSPLP HPESTTSDEK EVPLAQTAQP TSAIVRPASL QVPNVLLTSS DSSVIIQQAV P
Positive Samples: Lovo, MCF7, SW480, K-562; Cellular Location: Cytoplasm, Mitochondrion outer membrane, Nucleus
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating 2; Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB-2; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; MXBP protein; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354
Gene Aliases: Atf-2; ATF2; CRE-BP; CRE-BP1; CREB-2; CREB2; CREBP1; D130078H02Rik; D18875; HB16; mXBP; Tg(Gzma-Klra1)7Wum; TREB7
UniProt ID: (Human) P15336, (Rat) Q00969, (Mouse) P16951
Entrez Gene ID: (Human) 1386, (Rat) 81647, (Mouse) 11909
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
histone acetyltransferase activity
protein binding
cAMP response element binding protein binding
protein kinase binding
cAMP response element binding
metal ion binding
protein heterodimerization activity
DNA binding
sequence-specific DNA binding
nucleic acid binding
transferase activity
basic leucine zipper transcription factor
negative regulation of transcription from RNA polymerase II promoter
outflow tract morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to osmotic stress
cellular response to DNA damage stimulus
response to water deprivation
histone acetylation
intra-S DNA damage checkpoint
positive regulation of transforming growth factor beta2 production
positive regulation of neuron apoptotic process
fat cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of epithelial cell proliferation
regulation of sequence-specific DNA binding transcription factor activity
positive regulation of sequence-specific DNA binding transcription factor activity
adipose tissue development
amelogenesis
positive regulation of mitochondrial membrane permeability involved in apoptotic process
transcription, DNA-templated
Performance Guarantee
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