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ATF3 Antibody (PA5-77870) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of ATF3 was performed in HCT116 cells, irradiated (right) or non-irradiated (left) with 100 J/m2 UVC with 8hr recovery, fixed in 4% paraformaldehyde at RT for 5 min. Green: ATF3 Polyclonal Antibody (Product # PA5-77870) diluted at 1:500. Blue: Hoechst 33342 staining.
产品信息
PA5-77870
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Full length human ATF3 Recombinant protein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735491
产品详细信息
Positive Control: HeLa, HCT116(UVC-treated 10 J/m2 and recover for 8hr)
Predicted Reactivity: Mouse (95%), Rat (95%), Xenopus laevis (80%), Rhesus Monkey (99%), Bovine (95%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
ATF3 (Activating transcription factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements. An alternatively spliced form of ATF3 (ATF3 delta Zip) encodes a truncated form ATF3 protein lacking the leucine zipper protein-dimerization motif and does not bind to DNA. In contrast to ATF3, ATF3 delta Zip stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. Three transcript variants encoding two different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activating transcription factor 3; ATF 3; cAMP-dependent transcription factor ATF-3; Cyclic AMP-dependent transcription factor ATF-3
基因别名: ATF3
UniProt ID: (Human) P18847
Entrez Gene ID: (Human) 467
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
identical protein binding
protein homodimerization activity
transcription regulatory region DNA binding
protein heterodimerization activity
basic leucine zipper transcription factor
negative regulation of transcription from RNA polymerase II promoter
gluconeogenesis
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
cellular response to amino acid starvation
skeletal muscle cell differentiation
PERK-mediated unfolded protein response
positive regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance
negative regulation of ERK1 and ERK2 cascade
positive regulation of TRAIL-activated apoptotic signaling pathway
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
