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ATG5 Antibody (PA5-29694) in ICC/IF
ATG5 Polyclonal Antibody detects ATG5 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50 µM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG5 protein stained by ATG5 Polyclonal Antibody (Product # PA5-29694) diluted at 1:1,000. Red: Phalloidin, a F-actin marker.
产品信息
PA5-29694
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 64 and 275 of Human APG5
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2547168
产品详细信息
Recommended positive controls: NT2D1, PC-3, HeLa, HeLa (chloroquine treatment for 24hr).
Predicted reactivity: Mouse (95%), Xenopus laevis (81%), Chicken (87%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The APG5-APG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Diseases associated with ATG5 dysfunction include spinocerebellar ataxia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: APG 5; APG5 autophagy 5 like (APG5L); APG5-like; Apoptosis-specific protein; Apoptosis-specific protein (ASP); ATG5 autophagy related 5 homolog; Autophagy protein 5; autophagy-related 5
基因别名: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; ATG5; Atg5l; AW319544; C88337; hAPG5; Paddy
UniProt ID: (Human) Q9H1Y0, (Mouse) Q99J83
Entrez Gene ID: (Human) 9474, (Mouse) 11793
autophagosome assembly
mitophagy
blood vessel remodeling
regulation of cytokine secretion involved in immune response
C-terminal protein lipidation
autophagy
apoptotic process
cellular response to nitrogen starvation
response to fungus
macroautophagy
cellular homeostasis
antigen processing and presentation of endogenous antigen
negative regulation of protein ubiquitination
aggrephagy
negative stranded viral RNA replication
vasodilation
response to drug
negative regulation of apoptotic process
post-translational protein modification
nucleophagy
negative thymic T cell selection
innate immune response
otolith development
regulation of release of sequestered calcium ion into cytosol
ventricular cardiac muscle cell development
heart contraction
negative regulation of cell death
protein lipidation involved in autophagosome assembly
positive regulation of mucus secretion
regulation of cilium assembly
positive regulation of mitophagy
negative regulation of reactive oxygen species metabolic process
negative regulation of histone H4-K16 acetylation
immune system process
regulation of reactive oxygen species metabolic process
