Invitrogen

ATP Citrate Lyase Monoclonal Antibody (5F8D11)

ATP Citrate Lyase Antibody in Immunocytochemistry (ICC/IF)

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ATP Citrate Lyase Antibody (MA5-17027) in ICC/IF

Immunofluorescence analysis of HeLa cells using ACLY monoclonal antibody (Product # MA5-17027) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

ATP Citrate Lyase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP Citrate Lyase Antibody in Western Blot (WB)

ATP Citrate Lyase Antibody in Flow Cytometry (Flow)

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产品信息

MA5-17027

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:50

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

5F8D11

抗原

Purified recombinant fragment of human ACLY (amino acids: 306-502 ) expressed in E. Coli.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2538499

产品详细信息

MA5-17027 targets ACLY in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human, Mouse, Non-human primate, and Rat samples.

The MA5-17027 immunogen is purified recombinant fragment of human ACLY (amino acids: 306-502 ) expressed in E. Coli.

MA5-17027 detects ACLY which has a predicted molecular weight of approximately 125kDa.

靶标信息

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lactate Rewires Lipid Metabolism and Sustains a Metabolic-Epigenetic Axis in Prostate Cancer.

Cancer research

Ippolito L,Comito G,Parri M,Iozzo M,Duatti A,Virgilio F,Lorito N,Bacci M,Pardella E,Sandrini G,Bianchini F,Damiano R,Ferrone L,la Marca G,Serni S,Spatafora P,Catapano CV,Morandi A,Giannoni E,Chiarugi P

MA5-17027 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that stromal-derived lactate induces accumulation of lipid droplets, stimulates epigenetic rewiring, and fosters metastatic potential in prostate cancer.