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ATP Citrate lyase Antibody (711766) in WB
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of A549 (Lane 1), HeLa (Lane 2), NCI-H1975 (Lane 3), PC-3 (Lane 4), SK-OV-3 (Lane 5), A-431 (Lane 6), COS-7 (Lane 7), Jurkat (Lane 8), F9 (Lane 9) and tissue extracts of Mouse Lung (Lane 10). The blots were probed with Anti-ATP Citrate lyase Recombinant Rabbit Superclonal™ Antibody (Product # 711766, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:400... View More
产品信息
711766
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
8HCLC
抗原
Protein corresponding to human ATP Citrate Lyase [aa1-aa425]
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2716907
产品详细信息
This antibody predicted to react with Monkey, Sheep, Cat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ACL; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; Citrate cleavage enzyme
基因别名: A730098H14Rik; ACL; ACLY; ATPCL; AW538652; CLATP
UniProt ID: (Human) P53396, (Mouse) Q91V92
Entrez Gene ID: (Human) 47, (Mouse) 104112
ATP citrate synthase activity
protein binding
ATP binding
metal ion binding
cofactor binding
nucleotide binding
catalytic activity
succinate-CoA ligase (ADP-forming) activity
transferase activity
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
transferase
acetyl-CoA biosynthetic process
citrate metabolic process
oxaloacetate metabolic process
fatty acid biosynthetic process
cholesterol biosynthetic process
lipid biosynthetic process
positive regulation of cellular metabolic process
long-chain fatty-acyl-CoA biosynthetic process
acetyl-CoA metabolic process
lipid metabolic process
metabolic process
cellular carbohydrate metabolic process
