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Invitrogen

ATP5A1 Monoclonal Antibody (15H4C4)

Name: ATP5A1 Monoclonal Antibody (15H4C4)
Brand: Invitrogen
SKU: 439800
Price: 6171.0 CNY

7个已发表图片

23 篇参考文献

查看其他22个ATP5A1抗体

说明书

实验方案

技术支持

货号 43-9800

CNY 6,171.00

100 µg

下单后预计有货时间 18-Apr-2025

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说明书

实验方案

技术支持

抗体检测数据 (5)
已发表图片 (7)

Application

ATP5A1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 12

ATP5A1 Antibody (43-9800) in ICC/IF

Immunofluorescence analysis of ATP5A1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATP5A1 Monoclonal Antibody (15H4C4) (Product # 43-9800) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a... View More

ATP5A1 Antibody in Flow Cytometry (Flow)

ATP5A1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

43-9800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1.0 µg/mL

查看15篇已发表文章 15篇已发表文章

免疫组化 (IHC)

1 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1-2 µg/mL

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

1 µg/mL

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Fruit fly, Human, Mouse, Rat

已发表种属

C. elegans, Fruit fly, Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

15H4C4

抗原

Bovine Complex V

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533548

产品详细信息

When performing IHC use heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Positive control: Human, bovine, mouse and rat heart mitochondria.

靶标信息

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

M1BP is an essential transcriptional activator of oxidative metabolism during Drosophila development.

Nature communications

Poliacikova G,Barthez M,Rival T,Aouane A,Luis NM,Richard F,Daian F,Brouilly N,Schnorrer F,Maurel-Zaffran C,Graba Y,Saurin AJ

Published figure using ATP5A1 monoclonal antibody (Product # 43-9800) in Western Blot

Fri Jun 02 00:00:00 UTC 2023

Inhibition of ATP synthase reverse activity restores energy homeostasis in mitochondrial pathologies.

The EMBO journal

Acin-Perez R,Benincá C,Fernandez Del Rio L,Shu C,Baghdasarian S,Zanette V,Gerle C,Jiko C,Khairallah R,Khan S,Rincon Fernandez Pacheco D,Shabane B,Erion K,Masand R,Dugar S,Ghenoiu C,Schreiner G,Stiles L,Liesa M,Shirihai OS

Published figure using ATP5A1 monoclonal antibody (Product # 43-9800) in Western Blot

Mon May 15 00:00:00 UTC 2023

Inhibition of ATP synthase reverse activity restores energy homeostasis in mitochondrial pathologies.

The EMBO journal

Published figure using ATP5A1 monoclonal antibody (Product # 43-9800) in Western Blot

Mon May 15 00:00:00 UTC 2023

Combining albumin deficiency and acute exercise reduces hepatic lipid droplet size in mice.

Lipids in health and disease

Zhang Y,Szramowski M,Sun S,Henderson GC

43-9800 was used in Western Blotting to investigate the combined effect of albumin deficiency and acute exercise on hepatic lipid droplets (LDs).

Wed Jun 21 00:00:00 UTC 2023

Coronaviral ORF6 protein mediates inter-organelle contacts and modulates host cell lipid flux for virus production.

The EMBO journal

Yue M,Hu B,Li J,Chen R,Yuan Z,Xiao H,Chang H,Jiu Y,Cai K,Ding B

43-9800 was used in Western Blot to find that ORF6 inserts into the LD lipid monolayer via its two amphipathic helices.

Mon Jul 03 00:00:00 UTC 2023

mTORC2 protects the heart from high-fat diet-induced cardiomyopathy through mitochondrial fission in Drosophila.

Frontiers in cell and developmental biology

Liu P,Chang K,Requejo G,Bai H

Published figure using ATP5A1 monoclonal antibody (Product # 43-9800) in Immunohistochemistry

Thu Sep 29 00:00:00 UTC 2022

mTORC2 protects the heart from high-fat diet-induced cardiomyopathy through mitochondrial fission in Drosophila.

Frontiers in cell and developmental biology

Liu P,Chang K,Requejo G,Bai H

Published figure using ATP5A1 monoclonal antibody (Product # 43-9800) in Immunohistochemistry

Thu Sep 29 00:00:00 UTC 2022

mt tRFs, New Players in MELAS Disease.

Frontiers in physiology

Meseguer S,Rubio MP

Published figure using ATP5A1 monoclonal antibody (Product # 43-9800) in Immunocytochemistry

Mon Mar 14 00:00:00 UTC 2022

mt tRFs, New Players in MELAS Disease.

Frontiers in physiology

Meseguer S,Rubio MP

Published figure using ATP5A1 monoclonal antibody (Product # 43-9800) in Immunocytochemistry

Mon Mar 14 00:00:00 UTC 2022

In vivo characterization of Drosophila golgins reveals redundancy and plasticity of vesicle capture at the Golgi apparatus.

Current biology : CB

Park SY,Muschalik N,Chadwick J,Munro S

43-9800 was used in Immunohistochemistry to show that ectopic forms can capture intra-Golgi transport vesicles, but strikingly, the cargo present in the vesicles captured by each golgin varies between tissues.

Mon Nov 07 00:00:00 UTC 2022

Bacterial peptidoglycan muropeptides benefit mitochondrial homeostasis and animal physiology by acting as ATP synthase agonists.

Developmental cell

Tian D,Han M

43-9800 was used in Western Blot to identify a role for muropeptides in supporting mitochondrial homeostasis, development, and behaviors in Caenorhabditis elegans.

Mon Feb 07 00:00:00 UTC 2022

Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila.

Frontiers in cell and developmental biology

Nilangekar K,Murmu N,Sahu G,Shravage BV

43-9800 was used in Immunohistochemistry to provide a valuable tool that can be utilized in designing genetic screens to identify novel regulators of autophagy and redox homeostasis during oogenesis.

Tue Sep 29 00:00:00 UTC 2020

Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses.

Journal of cell science

Deutschman E,Ward JR,Kumar A,Ray G,Welch N,Lemieux ME,Dasarathy S,Longworth MS

43-9800 was used in Western Blot to identify condensin II as novel regulators of mitochondrial respiration and mitochondrial stress responses.

Wed Nov 20 00:00:00 UTC 2019

Assays to Monitor Mitophagy in Drosophila.

Methods in molecular biology (Clifton, N.J.)

Tsapras P,Jacomin AC,Nezis IP

43-9800 was used in Immunohistochemistry to provide two protocols to prepare Drosophila samples for confocal and transmission electron microscopy for in vivo monitoring of mitophagy, a specific type of autophagy for the clearance of damaged or superfluous mitochondria from cells.

Thu Jun 06 00:00:00 UTC 2019

Mitochondrial Oxidative Phosphorylation defect in the Heart of Subjects with Coronary Artery Disease.

Scientific reports

Ait-Aissa K,Blaszak SC,Beutner G,Tsaih SW,Morgan G,Santos JH,Flister MJ,Joyce DL,Camara AKS,Gutterman DD,Donato AJ,Porter GA,Beyer AM

43-9800 was used in Western Blotting to study via PCR analysis to reveal an increase in mtDNA lesions and the frequency of mitochondrial common deletion, both established markers for impaired mitochondrial integrity in CAD compared to non-CAD patient samples.

Mon May 20 00:00:00 UTC 2019

Fascin Controls Metastatic Colonization and Mitochondrial Oxidative Phosphorylation by Remodeling Mitochondrial Actin Filaments.

Cell reports

Lin S,Huang C,Gunda V,Sun J,Chellappan SP,Li Z,Izumi V,Fang B,Koomen J,Singh PK,Hao J,Yang S

43-9800 was used in Western Blot to report that fascin, an actin-bundling protein, promotes lung cancer metastatic colonization by augmenting metabolic stress resistance and mitochondrial oxidative phosphorylation (OXPHOS).

Tue Sep 10 00:00:00 UTC 2019

SNX14 mutations affect endoplasmic reticulum-associated neutral lipid metabolism in autosomal recessive spinocerebellar ataxia 20.

Human molecular genetics

Bryant D,Liu Y,Datta S,Hariri H,Seda M,Anderson G,Peskett E,Demetriou C,Sousa S,Jenkins D,Clayton P,Bitner-Glindzicz M,Moore GE,Henne WM,Stanier P

43-9800 was used in Western Blotting to determine the role of SNX14 in endoplasmic reticulum-lipid droplet crosstalk.

Fri Jun 01 00:00:00 UTC 2018

MICU1 Confers Protection from MCU-Dependent Manganese Toxicity.

Cell reports

Wettmarshausen J,Goh V,Huang KT,Arduino DM,Tripathi U,Leimpek A,Cheng Y,Pittis AA,Gabaldón T,Mokranjac D,Hajnóczky G,Perocchi F

43-9800 was used in Western Blot to dissect the interdependence between the pore-forming subunit MCU and the calcium-sensing regulator MICU1.

Tue Nov 06 00:00:00 UTC 2018

Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase.

Cell

Qi B,Han M

43-9800 was used in Western Blot to reveal a distinct paradigm for the iron tug of war" between commensal bacteria and their hosts and an important mechanism for mitochondrial iron uptake and homeostasis."

Thu Oct 04 00:00:00 UTC 2018

Regulation of autophagy, mitochondrial dynamics, and cellular bioenergetics by 4-hydroxynonenal in primary neurons.

Autophagy

Dodson M,Wani WY,Redmann M,Benavides GA,Johnson MS,Ouyang X,Cofield SS,Mitra K,Darley-Usmar V,Zhang J

43-9800 was used in Western Blotting to show that mitochondria and autophagy are critical targets of 4-hydroxynonenal, and that the proteins targeted by 4-hydroxynonenal may change based on its concentration, leading to cellular dysfunction.

Tue Jun 04 00:00:00 UTC 2019

Reducing Lissencephaly-1 levels augments mitochondrial transport and has a protective effect in adult Drosophila neurons.

Journal of cell science

Vagnoni A,Hoffmann PC,Bullock SL

43-9800 was used in western blot to learn about augmentation of mitochondrial transport and protective effect in adult Drosophila neurons by reducing lissencephaly-1 levels

Fri Jan 01 00:00:00 UTC 2016

Downstream effects of plectin mutations in epidermolysis bullosa simplex with muscular dystrophy.

Acta neuropathologica communications

Winter L,Türk M,Harter PN,Mittelbronn M,Kornblum C,Norwood F,Jungbluth H,Thiel CT,Schlötzer-Schrehardt U,Schröder R

43-9800 was used in western blot to study muscular dystrophy and the downstream effects of plectin mutations in epidermolysis bullosa simplex

Wed Apr 27 00:00:00 UTC 2016

Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells.

Free radical biology & medicine

Dun Y,Vargas J,Brot N,Finnemann SC

43-9800 was used in western blot to test if and how MsrA affects retinal pigment epithelium functionality.

Sun Dec 01 00:00:00 UTC 2013

A chemical cross-linking method for the analysis of binding partners of heat shock protein-90 in intact cells.

BioTechniques

Song S,Kole S,Bernier M

Sun Apr 01 00:00:00 UTC 2012

A chemical cross-linking method for the analysis of binding partners of heat shock protein-90 in intact cells.

BioTechniques

Song S,Kole S,Bernier M

Sun Apr 01 00:00:00 UTC 2012

生物信息学

蛋白别名: alpha subunit ATP synthase isoform precursor (EC 3.6.1.34); ATP synthase alpha chain, mitochondrial; ATP synthase alpha subunit; ATP synthase alpha subunit precursor; ATP synthase F1 subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; epididymis secretory sperm binding protein Li 123m; iso; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2

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基因别名: AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5A2; ATP5AL2; ATP5F1A; ATPM; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM

UniProt ID: (Human) P25705, (Bovine) P19483, (Rat) P15999, (Mouse) Q03265

Entrez Gene ID: (Human) 498, (Bovine) 282578, (Rat) 65262, (Mouse) 11946

功能

protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding poly(A) RNA binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism ADP binding nucleotide binding hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances ATP synthase

参与通路

negative regulation of endothelial cell proliferation lipid metabolic process ATP biosynthetic process embryo development ATP hydrolysis coupled proton transport mitochondrial ATP synthesis coupled proton transport ATP synthesis coupled proton transport ATP metabolic process hydrogen ion transmembrane transport transport ion transport proton transport

ATP5A1 Monoclonal Antibody (15H4C4)

ATP5A1 Antibody (43-9800) in ICC/IF

产品信息

43-9800

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

ATP5A1 Monoclonal Antibody (15H4C4)

ATP5A1 Antibody (43-9800) in ICC/IF

产品信息

43-9800

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID