Invitrogen

AXIN2 Monoclonal Antibody (J.415.10)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 篇参考文献

查看其他12个AXIN2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (2)

图: 1 / 5

AXIN2 Antibody (MA5-15015) in WB

Western blot was performed using Anti-AXIN2 Monoclonal Antibody (J.415.10)(Product # MA5-15015) and a 94 kDa band corresponding to AXIN2 was observed across tissues tested. Tissue extracts (30 µg lysate) of Rat Brain (Lane 1), Rat Prostate (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and ... View More

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AXIN2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Rat Brain and Rat Prostate owing to their inherent genetic constitution. Relative expression of AXIN2 was observed in Rat Brain (High expressing) and Rat Prostate (Low expressing), as reported, using Anti-AXIN2 Monoclonal Antibody (J.415.10) (Product # MA5-15015) in Western Blot. {RE}

产品信息

MA5-15015

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:50

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

J.415.10

抗原

Synthetic peptide corresponding to residues surrounding Pro566 of human Axin2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

183 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10984109

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with Axin1.

靶标信息

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

仅用于科研。不用于诊断过程。未经明确授权不得转售。