Rockland

Acetylcholinesterase Polyclonal Antibody, HRP

产品信息

200-103-232-0100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,500

酶联免疫吸附实验 (ELISA)

1:2,000-1:10,000

免疫沉淀 (IP)

1:500-1:2,500

产品规格

种属反应

Bovine

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Acetylcholinesterase [Bovine Erythrocytes]

查看免疫原

偶联物

HRP HRP HRP

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Ion-exchange chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

内含物

0.01% gentamicin sulfate

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Acetylcholinesterase (Bovine Erythrocytes) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Acetylcholinesterase [Bovine Erythrocytes]. Cross reactivity against Acetylcholinesterase from other sources is unknown.

靶标信息

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.

仅用于科研。不用于诊断过程。未经明确授权不得转售。