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Actin Antibody (MA5-55245) in WB
Western blot analysis of lysates from Oryza sativa, using Actin Recombinant Monoclonal Antibody (Product # MA5-55245) secondary antibody at 1:10,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
MA5-55245
产品规格
种属反应
Plant
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
5M5Z8
抗原
A synthetic peptide corresponding to a sequence within amino acids 1-100 of Actin(Plant Specific).
偶联物
形式
Liquid
浓度
2.12 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Dry ice (international)
产品详细信息
Immunogen Sequence: MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA QSKRGILTLK YPIEHGIVNN WDDMEKIWHH TFYNELRVAP
靶标信息
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: alpha-sma; Atcb; pan actin; RP5-1068B5.2
