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Adenosine A1 Receptor Polyclonal Antibody

Adenosine A1 Receptor Antibody in Immunocytochemistry (ICC/IF)

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Adenosine A1 Receptor Antibody (AAR-006-200UL) in ICC/IF

Expression of AdenosineA1Receptor in rat DRG primary culture - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture. A. Staining of DRG cells with Anti-AdenosineA1Receptor Antibody (#AAR-006) (1:100), followed by goat Anti-rabbit AlexaFluor-555 secondary Antibody . B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.C. Mer... View More

Adenosine A1 Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F))

Adenosine A1 Receptor Antibody in Western Blot (WB)

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产品信息

AAR-006-200UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR, 3rd intracellular loop

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.85 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

靶标信息

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。