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Adiponectin Antibody (500-P239-1MG) in WB
Western Blot: To detect Human Adiponectin by Western Blot analysis Adiponectin Polyclonal Antibody (Product # 500-P239-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adiponectin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P239-1MG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Hi-5 Insect cells-derived Recombinant Human Adiponectin
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929985
产品详细信息
AA Sequence of recombinant protein: RGHHHHHHHH ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Adiponectin. Anti-Human Adiponectin-specific antibody was purified by affinity chromatography employing an immobilized Human Adiponectin matrix.
Sandwich ELISA: To detect Human Adiponectin by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Adiponectin (500-P239BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human Adiponectin.
Western Blot: To detect Human Adiponectin by Western Blot analysi this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adiponectin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.
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生物信息学
蛋白别名: 30 kDa adipocyte complement-related protein; ACRP30; Adipocyte complement-related 30 kDa protein; Adipocyte, C1q and collagen domain-containing protein; Adiponectin; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADPIOQ; apM-1; Gelatin-binding protein; gelatin-binding protein 28
基因别名: ACDC; ACRP30; ADIPOQ; ADIPQTL1; ADPN; APM-1; APM1; GBP28
UniProt ID: (Human) Q15848
Entrez Gene ID: (Human) 9370
response to hypoxia
positive regulation of protein phosphorylation
glucose metabolic process
generation of precursor metabolites and energy
fatty acid beta-oxidation
response to nutrient
circadian rhythm
response to sucrose
response to glucose
positive regulation of signal transduction
negative regulation of platelet-derived growth factor receptor signaling pathway
positive regulation of protein kinase A signaling
negative regulation of macrophage derived foam cell differentiation
negative regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of cholesterol efflux
regulation of glucose metabolic process
response to activity
negative regulation of smooth muscle cell migration
fatty acid oxidation
negative regulation of cell migration
negative regulation of granulocyte differentiation
negative regulation of protein autophosphorylation
positive regulation of cellular protein metabolic process
negative regulation of tumor necrosis factor production
positive regulation of interleukin-8 production
cellular response to insulin stimulus
positive regulation of myeloid cell apoptotic process
adiponectin-activated signaling pathway
negative regulation of heterotypic cell-cell adhesion
low-density lipoprotein particle clearance
response to tumor necrosis factor
cellular response to drug
glucose homeostasis
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of MAP kinase activity
response to ethanol
negative regulation of fat cell differentiation
negative regulation of macrophage differentiation
negative regulation of low-density lipoprotein particle receptor biosynthetic process
negative regulation of gluconeogenesis
negative regulation of blood pressure
positive regulation of blood pressure
negative regulation of transcription, DNA-templated
positive regulation of fatty acid metabolic process
positive regulation of glucose import
negative regulation of hormone secretion
negative regulation of smooth muscle cell proliferation
negative regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of phagocytosis
negative regulation of synaptic transmission
brown fat cell differentiation
protein homooligomerization
response to glucocorticoid
membrane depolarization
membrane hyperpolarization
protein heterotrimerization
negative regulation of ERK1 and ERK2 cascade
response to linoleic acid
detection of oxidative stress
cellular response to cAMP
positive regulation of monocyte chemotactic protein-1 production
cellular response to epinephrine stimulus
protein localization to plasma membrane
negative regulation of intracellular protein transport
negative regulation of receptor binding
negative regulation of DNA biosynthetic process
positive regulation of glycogen (starch) synthase activity
positive regulation of metanephric glomerular visceral epithelial cell development
positive regulation of cAMP-dependent protein kinase activity
positive regulation of renal albumin absorption
negative regulation of platelet-derived growth factor receptor-alpha signaling pathway
negative regulation of metanephric mesenchymal cell migration
