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Aggrecan Antibody (MA5-42646) in IHC (P)
Immunohistochemistry analysis of Aggrecan in paraffin-embedded mouse bone marrow. Samples were incubated with Aggrecan Monoclonal antibody (Product # MA5-42646) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
产品信息
MA5-42646
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
6L4T2
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 761-861 of human Aggrecan (P16112).
偶联物
Unconjugated
形式
Liquid
浓度
1.5 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2911787
产品详细信息
The target is usually found in the following locations: Secreted, extracellular matrix, extracellular space.
Immunogen sequence: WPPTGAATEE STEGPSATEV PSASEEPSPS EVPFPSEEPS PSEEPFPSVR PFPSVELFPS EEPFPSKEPS PSEEPSASEE PYTPSPPVPS WTELPSSGEE S
靶标信息
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: aggrecan 1; Aggrecan core protein; aggrecan, structural proteoglycan of cartilage; Cartilage-specific proteoglycan core protein; CSPCP
基因别名: Acan; Agc; Agc1; b2b183Clo; cmd; Cspg1
UniProt ID: (Rat) P07897, (Mouse) Q61282
Entrez Gene ID: (Rat) 58968, (Mouse) 11595
cartilage condensation
ossification
chondrocyte development
cell adhesion
central nervous system development
response to radiation
response to mechanical stimulus
response to gravity
response to glucose
multicellular organism aging
response to acidic pH
response to organic cyclic compound
spinal cord development
proteoglycan biosynthetic process
collagen fibril organization
negative regulation of cell migration
response to drug
chondroblast differentiation
cellular response to growth factor stimulus
skeletal system development
