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Agrin Antibody (PA5-37121) in IHC (P)
Immunohistochemistry analysis of Agrin in paraffin-embedded human colon carcinoma tissue (secreted staining) and negative control (right, with PBS only). Samples were incubated with Agrin polyclonal antibody (Product # PA5-37121) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
产品信息
PA5-37121
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to residues in human AGRN
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2553917
产品详细信息
This antibody detects endogenous protein at a molecular weight of 217 kDa.
Purity is >95% by SDS-PAGE.
靶标信息
Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated "y" and "z" display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Agrin; agrin proteoglycan; RP23-139J18.2
基因别名: AGR; AGRIN; AGRN; CMS8; CMSPPD; nmf380
UniProt ID: (Human) O00468, (Rat) P25304
Entrez Gene ID: (Human) 375790, (Mouse) 11603, (Rat) 25592
dystroglycan binding
structural constituent of cytoskeleton
calcium ion binding
protein binding
sialic acid binding
chondroitin sulfate binding
laminin binding
heparan sulfate proteoglycan binding
acetylcholine receptor regulator activity
BMP binding
ATPase inhibitor activity
ion channel binding
transforming growth factor beta binding
protease inhibitor
retinoid metabolic process
glycosaminoglycan biosynthetic process
glycosaminoglycan catabolic process
cytoskeleton organization
signal transduction
G-protein coupled acetylcholine receptor signaling pathway
extracellular matrix organization
glycosaminoglycan metabolic process
receptor clustering
positive regulation of GTPase activity
clustering of voltage-gated sodium channels
positive regulation of synaptic growth at neuromuscular junction
positive regulation of transcription from RNA polymerase II promoter
synapse organization
positive regulation of filopodium assembly
regulation of protein phosphorylation
positive regulation of protein phosphorylation
plasma membrane organization
chemical synaptic transmission
multicellular organism development
synapse assembly
neuromuscular junction development
regulation of synaptic growth at neuromuscular junction
regulation of receptor activity
cell differentiation
positive regulation of protein binding
regulation of GTPase activity
positive regulation of neuron apoptotic process
neurotransmitter receptor metabolic process
protein heterotetramerization
regulation of cardiac muscle contraction
positive regulation of protein tyrosine kinase activity
regulation of microtubule cytoskeleton organization
skeletal muscle acetylcholine-gated channel clustering
regulation of cardiac muscle cell membrane potential
regulation of axon guidance
negative regulation of sodium ion export from cell
negative regulation of sodium:potassium-exchanging ATPase activity
positive regulation of protein geranylgeranylation
