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Alcohol Dehydrogenase Antibody (200-4143-0100) in WB
Western Blot of Alcohol Dehydrogenase Antibody. Lane 1: Alcohol Dehydrogenase. Load: 50ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase Conjugated Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 Blocking Buffer for 30 min at RT. Predicted: 37kDa/Observed size: 45kDa.
产品信息
200-4143-0100
产品规格
种属反应
Yeast
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Alcohol Dehydrogenase [Yeast]
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-ALCOHOL DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.
靶标信息
This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ADHI; Alcohol dehydrogenase 1; Alcohol dehydrogenase I; NADH-dependent methylglyoxal reductase; YADH-1
基因别名: ADC1; ADH1; O0947; YOL086C
UniProt ID: (Baker's yeast) P00330
Entrez Gene ID: (Baker's yeast) 854068
