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Invitrogen
Alpha-Smooth Muscle Actin Monoclonal Antibody (0.N.5)
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Alpha-Smooth Muscle Actin Antibody (MA1-26017) in ICC/IF
Immunofluorescence analysis of Alpha Smooth Muscle Actin was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Alpha-Smooth Muscle Actin Monoclonal Antibody (0.N.5) (Product # MA1-26017) at 1:100 dilutio... View More
产品信息
MA1-26017
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
0.N.5
抗原
Synthetic peptide corresponding to the N-terminal residues M(1) C E E E D S T A L(10) of alpha smooth muscle Actin.
偶联物
形式
Liquid
浓度
0.04 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_779145
产品详细信息
It does not react with fibroblasts, striated muscle, or myocardium.
Positive control of smooth muscle or leiomyosarcoma suggested.
靶标信息
Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Actin Alpha 2 Smooth Muscle Aorta; Actin Vascular Smooth Muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Alpha Cardiac Actin; Alpha-actin-2; alpha-cardiac actin; Aortic Smooth Muscle; asma; Cell growth-inhibiting gene 46 protein; Growth Inhibiting Gene 46; smooth muscle; smooth muscle alpha-actin
基因别名: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; MYMY5; SMalphaA
UniProt ID: (Human) P62736, (Rat) P62738, (Mouse) P62737
Entrez Gene ID: (Human) 59, (Rat) 81633, (Mouse) 11475
