Invitrogen

Alpha-Smooth Muscle Actin Recombinant Rabbit Monoclonal Antibody (17H19L35)

Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry (ICC/IF)

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Alpha-Smooth Muscle Actin Antibody (701457) in ICC/IF

Immunofluorescent analysis of Smooth muscle actin was done on 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Smooth muscle actin Recombinant Rabbit Monoclonal Antibody (Product # 701457) at 2 µg-4 µg in 1% BSA a... View More

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Alpha-Smooth Muscle Actin Antibody in Western Blot (WB)

Alpha-Smooth Muscle Actin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (IHC)

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产品信息

701457

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫细胞化学 (ICC/IF)

2-4 µg

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

17H19L35

抗原

Peptide corresponding to amino acids 2-10 of human Smooth Muscle Actin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532485

产品详细信息

This antibody is predicted to react with bovine, canine, rabbit, rat and porcine based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Pregnane X Receptor and Indole-3-Propionic Acid Shape the Intestinal Mesenchyme to Restrain Inflammation and Fibrosis.

Cellular and molecular gastroenterology and hepatology

Flannigan KL,Nieves KM,Szczepanski HE,Serra A,Lee JW,Alston LA,Ramay H,Mani S,Hirota SA

Published figure using Alpha-Smooth Muscle Actin recombinant monoclonal antibody (Product # 701457) in Immunocytochemistry

Thu Feb 23 00:00:00 EST 2023

The Pregnane X Receptor and Indole-3-Propionic Acid Shape the Intestinal Mesenchyme to Restrain Inflammation and Fibrosis.

Cellular and molecular gastroenterology and hepatology

Flannigan KL,Nieves KM,Szczepanski HE,Serra A,Lee JW,Alston LA,Ramay H,Mani S,Hirota SA

Published figure using Alpha-Smooth Muscle Actin recombinant monoclonal antibody (Product # 701457) in Immunocytochemistry

Thu Feb 23 00:00:00 EST 2023

Multiple benign fibrous histiocytomas of the mandible: A case report and review of the literature.

Experimental and therapeutic medicine

Wang Y,Huang Y,Cai WX,Tao Q

701457 was used in Immunohistochemistry to suggest that BFH has a risk of recurrence after transoral curettage. Regular follow-up is advised to detect tumor recurrence after the surgery of transoral curettage.

Thu Sep 01 00:00:00 EDT 2022

Single Cell Analysis of Cultivated Fibroblasts from Chronic Pancreatitis and Pancreatic Cancer Patients.

Cells

Sunami Y,Chen Y,Trojanowicz B,Sommerer M,Hämmerle M,Eils R,Kleeff J

Published figure using Alpha-Smooth Muscle Actin recombinant monoclonal antibody (Product # 701457) in Immunocytochemistry

Fri Aug 19 00:00:00 EDT 2022

Single Cell Analysis of Cultivated Fibroblasts from Chronic Pancreatitis and Pancreatic Cancer Patients.

Cells

Sunami Y,Chen Y,Trojanowicz B,Sommerer M,Hämmerle M,Eils R,Kleeff J

Published figure using Alpha-Smooth Muscle Actin recombinant monoclonal antibody (Product # 701457) in Immunocytochemistry

Fri Aug 19 00:00:00 EDT 2022

L-carnitine suppresses transient receptor potential vanilloid type 1 activity and myofibroblast transdifferentiation in human corneal keratocytes.

Laboratory investigation; a journal of technical methods and pathology

Turan E,Valtink M,Reinach PS,Skupin A,Luo H,Brockmann T,Ba Salem MHO,Pleyer U,Mergler S

701457 was used in Immunocytochemistry to investigated if L-carnitine can mediate inhibition of the fibrotic response through suppression of TRPV1 activation in human corneal keratocytes.

Tue Jun 01 00:00:00 EDT 2021

RNA Interference Targeting Liver Angiopoietin-Like Protein 3 Protects from Nephrotic Syndrome in a Rat Model Via Amelioration of Pathologic Hypertriglyceridemia.

The Journal of pharmacology and experimental therapeutics

Zhao Y,Goto M,Vaziri ND,Khazaeli M,Liu H,Farahanchi N,Khanifar E,Farzaneh T,Haslett PA,Moradi H,Soundarapandian MM

701457 was used in Western Blotting to present an alternate therapeutic approach by using siRNA targeting Angptl3 expressed in hepatocytes.

Mon Mar 01 00:00:00 EST 2021

A uro-protective agent with restorative actions on urethral and striated muscle morphology.

World journal of urology

Birder LA,Wolf-Johnston A,Wein AJ,Grove-Sullivan M,Stoltz D,Watkins S,Newman D,Dmochowski RR,Jackson EK

701457 was used in Western Blotting to support the concept that 8-AG will reverse age-induced lower urinary tract disorders such as UI.

Thu Jul 01 00:00:00 EDT 2021

Purine nucleoside phosphorylase inhibition ameliorates age-associated lower urinary tract dysfunctions.

JCI insight

Birder LA,Wolf-Johnston A,Wein AJ,Cheng F,Grove-Sullivan M,Kanai AJ,Watson AM,Stoltz D,Watkins SC,Robertson AM,Newman D,Dmochowski RR,Jackson EK

701457 was used in Western Blotting to demonstrate that 8-AG has translational potential for treating age-associated LUT dysfunctions and resultant syndromes in humans.

Thu Oct 15 00:00:00 EDT 2020

Purine nucleoside phosphorylase inhibition ameliorates age-associated lower urinary tract dysfunctions.

JCI insight

Birder LA,Wolf-Johnston A,Wein AJ,Cheng F,Grove-Sullivan M,Kanai AJ,Watson AM,Stoltz D,Watkins SC,Robertson AM,Newman D,Dmochowski RR,Jackson EK

701457 was used in Western Blotting to demonstrate that 8-AG has translational potential for treating age-associated LUT dysfunctions and resultant syndromes in humans.

Thu Oct 15 00:00:00 EDT 2020

TFAP2C facilitates somatic cell reprogramming by inhibiting c-Myc-dependent apoptosis and promoting mesenchymal-to-epithelial transition.

Cell death & disease

Wang Y,Chen S,Jiang Q,Deng J,Cheng F,Lin Y,Cheng L,Ye Y,Chen X,Yao Y,Zhang X,Shi G,Dai L,Su X,Peng Y,Deng H

701457 was used in Immunocytochemistry to demonstrate Tfap2c is induced during the generation of iPSCs from mouse fibroblasts and acts as a facilitator for iPSCs formation.

Thu Jun 25 00:00:00 EDT 2020

Eugenol, a potential schistosomicidal agent with anti-inflammatory and antifibrotic effects against Schistosoma mansoni, induced liver pathology.

Infection and drug resistance

El-Kady AM,Ahmad AA,Hassan TM,El-Deek HEM,Fouad SS,Althagfan SS

701457 was used in Immunohistochemistry to investigate the possible antischistosomal effect of eugenol on Schistosoma mansoni.

Fri Apr 08 00:00:00 EDT 2022

CD98 regulates vascular smooth muscle cell proliferation in atherosclerosis.

Atherosclerosis

Baumer Y,McCurdy S,Alcala M,Mehta N,Lee BH,Ginsberg MH,Boisvert WA

701457 was used in immunohistochemistry to ask if CD98 heavy chain expressed on vascular smooth muscle cells plays a role in shaping the morphology of atherosclerotic plaques by regulating vascular smooth muscle cell function

Sun Jan 01 00:00:00 EST 2017

Severe pulmonary hypertension in aging female apolipoprotein E-deficient mice is rescued by estrogen replacement therapy.

Biology of sex differences

Umar S,Partow-Navid R,Ruffenach G,Iorga A,Moazeni S,Eghbali M

Published figure using Smooth Muscle Actin monoclonal antibody (Product # 701457) in Immunofluorescence

Wed Jun 06 00:00:00 EDT 2018

生物信息学

蛋白别名: Actin Alpha 2 Smooth Muscle Aorta; Actin Vascular Smooth Muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Alpha Cardiac Actin; Alpha-actin-2; alpha-cardiac actin; Aortic Smooth Muscle; asma; Cell growth-inhibiting gene 46 protein; Growth Inhibiting Gene 46; smooth muscle; smooth muscle alpha-actin

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基因别名: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; MYMY5; SMalphaA

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UniProt ID: (Human) P62736, (Rat) P62738, (Mouse) P62737

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Entrez Gene ID: (Human) 59, (Rat) 81633, (Mouse) 11475

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功能

ATP binding protein kinase binding nucleotide binding protein binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

参与通路

muscle contraction regulation of blood pressure response to virus positive regulation of gene expression vascular smooth muscle contraction glomerular mesangial cell development mesenchyme migration