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Angiopoietin 1 Antibody (MA5-32279) in WB
Western blot was performed using Anti-Angiopoietin 1 Recombinant Rabbit Monoclonal Antibody (SN67-02) (Product # MA5-32279) and a 60kDa band corresponding to Angiopoietin-1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), HUVEC (Lane 2), MDA-MB-231 (Lane 3), iPSC (Lane 4), MCF 10A (Lane 5), Raji (Lane 6), Mouse Testis (Lane 7), Rat Testis (Lane 8) and Mouse Liver (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then tr... View More
产品信息
MA5-32279
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SN67-02
抗原
Synthetic peptide within Human Angiopoietin 1 aa 249-300
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2809565
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ANG-1; Angiopoietin-1
基因别名: 1110046O21Rik; AGP1; AGPT; Agpt1; Ang-1; ANG1; ANGPT1; KIAA0003
UniProt ID: (Human) Q15389, (Mouse) O08538, (Rat) O35460
Entrez Gene ID: (Human) 284, (Mouse) 11600, (Rat) 89807
MAPK cascade
in utero embryonic development
negative regulation of protein phosphorylation
regulation of endothelial cell proliferation
sprouting angiogenesis
positive regulation of receptor internalization
negative regulation of cytokine secretion involved in immune response
negative regulation of cell adhesion
activation of transmembrane receptor protein tyrosine kinase activity
positive regulation of endothelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
regulation of skeletal muscle satellite cell proliferation
hemopoiesis
cell differentiation
heparin biosynthetic process
positive regulation of protein ubiquitination
cell-substrate adhesion
regulation of tumor necrosis factor production
positive regulation of peptidyl-serine phosphorylation
protein localization to cell surface
negative regulation of protein import into nucleus
negative regulation of apoptotic process
negative regulation of vascular permeability
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of protein binding
negative regulation of neuron apoptotic process
positive regulation of blood vessel endothelial cell migration
positive regulation of GTPase activity
positive regulation of cell adhesion
Tie signaling pathway
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
positive chemotaxis
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
glomerulus vasculature development
negative regulation of endothelial cell apoptotic process
regulation of macrophage migration inhibitory factor signaling pathway
angiogenesis
ovarian follicle development
patterning of blood vessels
vasculogenesis
endocardium morphogenesis
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
endoderm development
positive regulation of vascular endothelial growth factor receptor signaling pathway
protein homooligomerization
cardiac muscle tissue morphogenesis
vasculogenesis involved in coronary vascular morphogenesis
response to hypoxia
organ regeneration
response to vitamin B3
response to estrogen
