Invitrogen

Ankyrin B Monoclonal Antibody (2.2)

Ankyrin B Antibody in Immunocytochemistry (ICC/IF)

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Ankyrin B Antibody (33-3700) in ICC/IF

Immunofluorescent analysis of Ankyrin B was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Ankyrin B Mouse monoclonal Antibody (Product # 33-3700) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperat... View More

Ankyrin B Antibody in Immunohistochemistry (IHC)

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产品信息

33-3700

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

1:100-1:500

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酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Fruit fly, Hamster, Insect, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2.2

抗原

Synthetic peptide derived from the human ankyrin B protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533115

靶标信息

This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4. Multiple transcript variants encoding different isoforms have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Cantú syndrome mutation produces dual effects on KATP channels by disrupting ankyrin B regulation.

The Journal of general physiology

Crespo-García T,Rubio-Alarcón M,Cámara-Checa A,Dago M,Rapún J,Nieto-Marín P,Marín M,Cebrián J,Tamargo J,Delpón E,Caballero R

Published figure using Ankyrin B monoclonal antibody (Product # 33-3700) in Western Blot

Mon Jan 02 00:00:00 EST 2023

The L1 cell adhesion molecule constrains dendritic spine density in pyramidal neurons of the mouse cerebral cortex.

Frontiers in neuroanatomy

Murphy KE,Wade SD,Sperringer JE,Mohan V,Duncan BW,Zhang EY,Pak Y,Lutz D,Schachner M,Maness PF

33-3700 was used in Western Blotting to provide insight into the molecular mechanism of spine regulation and underscores the potential for this adhesion molecule to regulate cognitive and other L1-related functions that are abnormal in the L1 syndrome.

Sat May 13 00:00:00 EDT 2023

The L1 cell adhesion molecule constrains dendritic spine density in pyramidal neurons of the mouse cerebral cortex.

Frontiers in neuroanatomy

Murphy KE,Wade SD,Sperringer JE,Mohan V,Duncan BW,Zhang EY,Pak Y,Lutz D,Schachner M,Maness PF

Sat May 13 00:00:00 EDT 2023

PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B.

Nature communications

Bock T,Türk C,Aravamudhan S,Keufgens L,Bloch W,Rozsivalova DH,Romanello V,Nogara L,Blaauw B,Trifunovic A,Braun T,Krüger M

33-3700 was used in Immunohistochemistry to demonstrate if PERM1 interacts with the intracellular adaptor protein ankyrin B that connects the cytoskeleton to the plasma membrane.

Thu Aug 12 00:00:00 EDT 2021

PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B.

Nature communications

Bock T,Türk C,Aravamudhan S,Keufgens L,Bloch W,Rozsivalova DH,Romanello V,Nogara L,Blaauw B,Trifunovic A,Braun T,Krüger M

33-3700 was used in Immunohistochemistry to demonstrate if PERM1 interacts with the intracellular adaptor protein ankyrin B that connects the cytoskeleton to the plasma membrane.

Thu Aug 12 00:00:00 EDT 2021

Coupling of the Na+/K+-ATPase to Ankyrin B controls Na+/Ca2+ exchanger activity in cardiomyocytes.

Cardiovascular research

Skogestad J,Aronsen JM,Tovsrud N,Wanichawan P,Hougen K,Stokke MK,Carlson CR,Sjaastad I,Sejersted OM,Swift F

33-3700 was used in Western Blot to investigate the role of NKA binding to AnkB in cardiomyocytes.

Wed Jan 01 00:00:00 EST 2020

Activation of glial FGFRs is essential in glial migration, proliferation, and survival and in glia-neuron signaling during olfactory system development.

PloS one

Gibson NJ,Tolbert LP,Oland LA

33-3700 was used in immunocytochemistry to investigate the role of Fibroblast Growth Factor receptors in glial migration, proliferation, and survival.

Tue Sep 04 00:00:00 EDT 2012

Roles of specific membrane lipid domains in EGF receptor activation and cell adhesion molecule stabilization in a developing olfactory system.

PloS one

Gibson NJ,Tolbert LP,Oland LA

33-3700 was used in immunohistochemistry - paraffin section to review factors that contribute to the development of the olfactory system of Manduca sexta

Tue Sep 29 00:00:00 EDT 2009

Structural requirements for interaction of sodium channel beta 1 subunits with ankyrin.

The Journal of biological chemistry

Malhotra JD,Koopmann MC,Kazen-Gillespie KA,Fettman N,Hortsch M,Isom LL

Fri Jul 19 00:00:00 EDT 2002

Structural requirements for interaction of sodium channel beta 1 subunits with ankyrin.

The Journal of biological chemistry

Malhotra JD,Koopmann MC,Kazen-Gillespie KA,Fettman N,Hortsch M,Isom LL

Fri Jul 19 00:00:00 EDT 2002

生物信息学

蛋白别名: ANK-2; ank2; ankyrin 2, brain; ankyrin 3, epithelial; ankyrin B; ankyrin, brain; Ankyrin-2; ankyrin-2, nonerythrocytic; Ankyrin-B; Brain ankyrin; Non-erythroid ankyrin

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基因别名: AI835472; ANK-2; ANK2; Ank3; ANKB; AW491075; brank-2; LQT4

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UniProt ID: (Human) Q01484, (Mouse) Q8C8R3

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Entrez Gene ID: (Human) 287, (Mouse) 109676, (Rat) 362036

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功能