Invitrogen

Ankyrin G Monoclonal Antibody (N106/20), PerCP

Ankyrin G Antibody in Immunocytochemistry (ICC/IF)

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Ankyrin G Antibody (MA5-45542) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Ankyrin G monoclonal antibody (Product # MA5-45542) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 fo... View More

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产品信息

MA5-45542

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

N106/20

抗原

Fusion protein 1000 C-terminal amino acids of human Ankyrin G (also known as ANK-3 or ankyrin-3) encompassing all of Ankyrin G with the exception of Ankyrin repeats (aa 3377-4377).

偶联物

PerCP PerCP PerCP

激发/发射光谱

482/675 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931996

产品详细信息

1 µg/mL of MA5-45542 was sufficient for detection of Ankyrin-G in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects >approximately 200kDa.

This antibody was formerly sold as clone S106-20.

靶标信息

Ankyrin-G is found in several tissues including brain where, due to tissue-specific RNA processing, it is expressed as two different isoforms, localized to axon initial segments and nodes of Ranvier. Ankyrin-G is also found at sites of epithelial cell-cell contact and in the intracellular membranes of some cells. Ankyrin-G isoforms contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following a globular head domain. Ankyrin-G is thought to participate in the maintenance and targeting of ion channels and cell adhesion molecules to the nodes of Ranvier and to axon initial segments.

仅用于科研。不用于诊断过程。未经明确授权不得转售。