Invitrogen

ApoA2 Polyclonal Antibody

查看其他18个ApoA2抗体

抗体检测数据 (2)

ApoA2 Antibody in Immunocytochemistry (ICC/IF)

ApoA2 Antibody in Immunocytochemistry (ICC/IF)

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ApoA2 Antibody (PA5-114863) in ICC/IF

Immunocytochemistry analysis of ApoA2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114863) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

ApoA2 Antibody in Immunocytochemistry (ICC/IF)

ApoA2 Antibody in Western Blot (WB)

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ApoA2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ApoA2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114863) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

ApoA2 Antibody in Western Blot (WB) — Western blot analysis of ApoA2 in HUVEC whole lysates. Samples were incubated with polyclonal antibody (Product # PA5-114863).

产品信息

PA5-114863

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human APOA2(Accession P02652), corresponding to amino acid residues R23-T73.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2899499

产品详细信息

Antibody detects endogenous levels of total APOA2.

靶标信息

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL, and VLDL particles of human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins ( especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease that measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: APO A2; Apo-AII; Apolipoprotein; Apolipoprotein A-II; Apolipoprotein A2; Apolipoprotein AII

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基因别名: Alp-2; Apo-AII; Apoa-2; ApoA-II; APOA2; APOAII; Hdl-1

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UniProt ID: (Human) P02652, (Rat) P04638, (Mouse) P09813

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Entrez Gene ID: (Human) 336, (Rat) 25649, (Mouse) 11807

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lipid transporter activity protein binding phospholipid binding high-density lipoprotein particle binding lipid binding cholesterol binding cholesterol transporter activity phosphatidylcholine binding apolipoprotein receptor binding protein homodimerization activity protein heterodimerization activity lipase inhibitor activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding apolipoprotein

参与通路

retinoid metabolic process acute inflammatory response negative regulation of cytokine secretion involved in immune response triglyceride metabolic process phosphatidylcholine biosynthetic process cholesterol metabolic process phospholipid catabolic process response to glucose positive regulation of cholesterol esterification negative regulation of very-low-density lipoprotein particle remodeling viral process protein oxidation peptidyl-methionine modification regulation of intestinal cholesterol absorption organ regeneration regulation of protein stability negative regulation of cholesterol transport cholesterol efflux phospholipid efflux triglyceride-rich lipoprotein particle remodeling low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly high-density lipoprotein particle clearance lipoprotein metabolic process lipoprotein biosynthetic process response to drug cholesterol homeostasis positive regulation of catalytic activity response to estrogen reverse cholesterol transport positive regulation of interleukin-8 biosynthetic process diacylglycerol catabolic process negative regulation of lipid catabolic process positive regulation of lipid catabolic process response to glucocorticoid negative regulation of lipase activity negative regulation of cholesterol import negative regulation of cholesterol transporter activity protein folding lipid transport cholesterol transport fatty acid metabolic process transport beta-glucoside transport