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Arginase 1 Antibody (PA5-18684) in ICC/IF
Immunocytochemistry analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Samples were incubated with Arginase 1 polyclonal antibody (Product # PA5-18684) using a dilution of 10 µg/mL for 1 hr followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa ... View More
产品信息
PA5-18684
产品规格
种属反应
Mouse,
Rat
已发表种属
Rat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide sequence (NHKPETDYLKPPK) corresponding to the C-terminus amino acids of Arg1 (aa 311-323).
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_10980832
产品详细信息
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
靶标信息
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: A-I; AI type I arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase
基因别名: AI; AI256583; Arg-1; Arg1; PGIF
UniProt ID: (Rat) P07824, (Mouse) Q61176
Entrez Gene ID: (Rat) 29221, (Mouse) 11846
urea cycle
liver development
positive regulation of endothelial cell proliferation
arginine metabolic process
female pregnancy
aging
response to wounding
response to herbicide
response to manganese ion
response to zinc ion
response to selenium ion
regulation of L-arginine import
response to amine
arginine catabolic process to ornithine
lung development
response to lipopolysaccharide
collagen biosynthetic process
response to vitamin A
response to vitamin E
response to drug
response to amino acid
response to peptide hormone
response to cadmium ion
response to steroid hormone
response to axon injury
response to methylmercury
mammary gland involution
maternal process involved in female pregnancy
protein homotrimerization
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to glucagon stimulus
cellular response to dexamethasone stimulus
cellular response to transforming growth factor beta stimulus
