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B3GAT1 Antibody (PA5-116449) in ICC/IF
Immunocytochemical analysis of B3GAT1 in SHSY5Y cells using a B3GAT1 polyclonal antibody (Product #PA5-116449). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
产品信息
PA5-116449
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide within Human B3GAT1 aa 285-334
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2901081
产品详细信息
Positive controls: 293 cell lysates, F9, SHSY5Y, Rat brain tissue, mouse brain tissue.
靶标信息
The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Beta-1,3-glucuronyltransferase 1; CD57; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT-P; GLCATP; GlcUAT-P; GLCUATP; Glucuronosyltransferase P; glucuronosyltransferase-P; LEU7 antigen; NK-1; NK1; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
基因别名: 0710007K08Rik; AI846286; B3GAT1; CD57; GlcAT-P; GLCATP; GLCUATP; Hnk-1; HNK1; LEU7; NK-1; NK1
UniProt ID: (Human) Q9P2W7, (Rat) O35789, (Mouse) Q9CW73
Entrez Gene ID: (Human) 27087, (Rat) 117108, (Mouse) 76898
