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Application
FIGURE: 1 / 2
BANF1 Antibody (PA5-20328) in WB
Western Blot Validation in Human Kidney Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: BANF1 Polyclonal Antibody (Product # PA5-20328) (A: 0.5 µg/mL and B: 1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Product Details
PA5-20328
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 15 amino acid peptide from near the carboxy terminus of human BANF1.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11153647
Product Specific Information
Despite its predicted molecular weight, BANF1 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is human kidney tissue lysate.
PA5-20328 can be used with blocking peptide PEP-0448.
Target Information
Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BANF1 also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BANF1 will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BANF1 is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Barrier-to-autointegration factor; Breakpoint cluster region protein 1; LAP2-binding protein 1
Gene Aliases: BAF; BANF1; BCRG1; BCRP1; D14S1460; L2bp1; L2bp1/Baf; NGPS
UniProt ID: (Human) O75531, (Mouse) Q542E6
Entrez Gene ID: (Human) 8815, (Mouse) 23825
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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