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BAP1 Antibody (PA5-105741) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded BAP1 in human Prostate carcinoma tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with BAP1 polyclonal antibody (Product # PA5-105741) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
产品信息
PA5-105741
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human BAP1(Accession Q92560), corresponding to amino acid residues L343-Q393.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2853924
产品详细信息
Antibody detects endogenous levels of total BAP1.
靶标信息
BRCA1-associated protein-1, or BAP1, interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns. BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4. 8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21. 3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas. Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Brca1 associated protein 1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 6; cerebral protein-13; DKFZp686N04275; FLJ35406; FLJ37180; Ubiquitin carboxyl-terminal hydrolase BAP1
基因别名: BAP1; HUCEP-13; hucep-6; KIAA0272; UCHL2
UniProt ID: (Human) Q92560, (Rat) D3ZHS6
Entrez Gene ID: (Human) 8314, (Rat) 306257
regulation of cell growth
cellular protein modification process
ubiquitin-dependent protein catabolic process
negative regulation of cell proliferation
protein deubiquitination
monoubiquitinated protein deubiquitination
monoubiquitinated histone H2A deubiquitination
regulation of cell cycle
protein K48-linked deubiquitination
positive regulation of protein targeting to mitochondrion
