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BID p15 Antibody (44-436G) in ICC/IF
Immunofluorescence analysis of BID (cleavage site 59/60) was done on 70% confluent log phase A549. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with BID (cleavage site 59/60) Rabbit polyclonal Antibody (Product # 44-436G) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) ... View More
产品信息
44-436G
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
The antiserum was produced against a chemically synthesized peptide derived from the 15 kDa caspase-8 cleavage product of mouse BID.
偶联物
形式
Liquid
纯化类型
Affinity chromatography
保存液
Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533653
靶标信息
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: apoptic d; apoptic death agonist; BH3-interacting domain death agonist; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p22 BID
基因别名: 2700049M22Rik; AI875481; AU022477; BID; FP497
UniProt ID: (Human) P55957, (Mouse) P70444
Entrez Gene ID: (Human) 637, (Mouse) 12122
release of cytochrome c from mitochondria
protein targeting to mitochondrion
activation of cysteine-type endopeptidase activity involved in apoptotic process
brain development
extrinsic apoptotic signaling pathway via death domain receptors
apoptotic mitochondrial changes
response to estradiol
positive regulation of protein oligomerization
positive regulation of protein homooligomerization
glial cell apoptotic process
regulation of cell proliferation
signal transduction in response to DNA damage
regulation of apoptotic process
positive regulation of apoptotic process
protein homooligomerization
neuron apoptotic process
establishment of protein localization to membrane
positive regulation of release of cytochrome c from mitochondria
hepatocyte apoptotic process
mitochondrial outer membrane permeabilization
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
regulation of G1/S transition of mitotic cell cycle
positive regulation of extrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
apoptotic process
regulation of protein oligomerization
extrinsic apoptotic signaling pathway
regulation of mitochondrial membrane permeability involved in apoptotic process
