Invitrogen

BNIP3 Recombinant Rabbit Monoclonal Antibody (8H14L2)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1个已发表图片

1 篇参考文献

查看其他15个BNIP3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

BNIP3 Antibody in Immunocytochemistry (ICC/IF)

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BNIP3 Antibody (701696) in ICC/IF

Immunofluorescence was performed on methanol fixed Hela cells treated with Cobalt Chloride (15 µm/ 48 hours), for detection of BNIP3 using Anti-BNIP3 Recombinant Rabbit Monoclonal Antibody (Product # 701696, 1-2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of BNIP3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountan... View More

BNIP3 Antibody in Immunohistochemistry (IHC)

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Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot analysis using BNIP3 Antibody (8H14L2), Recombinant Rabbit Monoclonal (Product # 701696) shows induction of BNIP3 expression in HeLa and PC-3 cell lines upon treatment with cobalt chloride and etoposide, respectively. {TM}

产品信息

701696

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1 µg/mL

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1-2 µg/mL

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

8H14L2

抗原

Protein corresponding to Human BNIP3 (aa 1-140)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2607981

产品详细信息

This antibody is predicted to react with Monkey, Bovine and Equine.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。