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Invitrogen
Bcl-X Monoclonal Antibody (2H12), eBioscience™
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 5
Bcl-X Antibody (14-6994-81) in ICC/IF
Immunofluorescence analysis of Bcl-X was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Bcl-X Monoclonal Antibody (2H12), eBioscience™ (Product # 14-6994-81) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conj... View More
产品信息
14-6994-81
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
2H12
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_468381
产品详细信息
Description: The 2H12 antibody reacts with human, mouse and rat Bcl-X. Bcl-X functions as a central repressor of several apoptotic signals.This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins.
Applications Reported: The 2H12 antibody has been reported for use in immunoblotting (WB).
Applications Tested: The 2H12 antibody has been tested by Western blot analysis of HeLa lysate and can be used at less than 2 µg/mL. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The protein encoded the BCL-X gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: anti-apoptosis regulatory protein; Apoptosis regulator Bcl-X; B cell lymphoma 2 like; B cell lymphoma like X; B-cell leukemia/lymphoma x; Bcl 2 like 1; bcl x; Bcl xL; BCL XL/S; bcl-2 L1; Bcl-2-like protein 1; bcl-xS; Bcl2-L-1; BCL2-like 1; BclxL; BclXS; DKFZp781P2092; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3
基因别名: Bcl(X)L; Bcl-X; Bcl-XL; BCL-XL/S; bcl2-L-1; BCL2L; BCL2L1; BCLX; Blc2l; PPP1R52
UniProt ID: (Human) Q07817, (Mouse) Q64373, (Rat) P53563
Entrez Gene ID: (Human) 598, (Mouse) 12048, (Rat) 24888
cytokinesis
ovarian follicle development
in utero embryonic development
release of cytochrome c from mitochondria
endocytosis
mitotic cell cycle checkpoint
germ cell development
spermatogenesis
cell proliferation
positive regulation of cell proliferation
male gonad development
intrinsic apoptotic signaling pathway in response to DNA damage
apoptotic mitochondrial changes
fertilization
suppression by virus of host apoptotic process
response to cytokine
growth
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
response to cycloheximide
regulation of mitochondrial membrane permeability
neuron apoptotic process
regulation of mitochondrial membrane potential
cellular process regulating host cell cycle in response to virus
mitochondrion morphogenesis
cellular response to amino acid stimulus
cellular response to alkaloid
cellular response to gamma radiation
apoptotic process in bone marrow
negative regulation of establishment of protein localization to plasma membrane
negative regulation of release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway in absence of ligand
hepatocyte apoptotic process
negative regulation of execution phase of apoptosis
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
response to ischemia
apoptotic process
response to radiation
response to virus
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of anoikis
response to hypoxia
response to oxidative stress
response to inorganic substance
response to organonitrogen compound
response to lead ion
response to organic cyclic compound
response to hydrogen peroxide
response to peptide hormone
response to electrical stimulus
