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Application
FIGURE: 1 / 6
Brachyury Antibody (PA5-46984) in ICC/IF
Immunocytochemistry analysis of Brachyury in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm. Samples were incubated in Brachyury polyclonal antibody (Product # PA5-46984) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was locali... View More
Product Details
PA5-46984
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human Brachyury Ser2-Glu202
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610378
Product Specific Information
In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Brachyury protein; brachyury-like 2; brachyury-like 3; low ratio; Protein T; RP11-459F1.1; T brachyury homolog; T, brachyury homolog; T-box transcription factor T
Gene Aliases: Bra; cou; D17Mit170; Low; Lr; me75; SAVA; T; T1; TBXT; TFT; Tl2; Tl3
UniProt ID: (Human) O15178, (Mouse) P20293
Entrez Gene ID: (Human) 6862, (Mouse) 20997
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor binding
RNA polymerase II activating transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
protein binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
vasculogenesis
somitogenesis
neural plate morphogenesis
neural tube closure
heart morphogenesis
positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation
transcription, DNA-templated
signal transduction
penetration of zona pellucida
mesoderm development
mesoderm migration involved in gastrulation
positive regulation of cell proliferation
anterior/posterior axis specification, embryo
notochord formation
signal transduction involved in regulation of gene expression
BMP signaling pathway
post-anal tail morphogenesis
embryonic skeletal system development
canonical Wnt signaling pathway
bone morphogenesis
SMAD protein signal transduction
determination of heart left/right asymmetry
cellular response to retinoic acid
primitive streak formation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
multicellular organism development
anatomical structure morphogenesis
anterior/posterior pattern specification
reproductive process
notochord development
positive regulation of transcription from RNA polymerase II promoter
cardiac muscle cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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