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Bub3 Antibody (PA5-29781) in ICC/IF
Immunofluorescence analysis of Bub3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Bub3 Polyclonal Antibody (Product # PA5-29781) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034)... View More
产品信息
PA5-29781
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 1 and 194 of Human BUB3
偶联物
Unconjugated
形式
Liquid
浓度
0.32 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2547255
产品详细信息
Recommended positive controls: Neuro 2A, Raw264.7, C2C12, PC-12, Rat2, 293T, H1299, A431, HeLa, HepG2.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (83%), Xenopus laevis (92%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3 mitotic checkpoint protein; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; mitotic checkpoint component; Mitotic checkpoint protein BUB3; OTTHUMP00000020682; testicular tissue protein Li 27; WD repeat type I transmembrane protein A72.5
基因别名: Aa2-050; AU019800; AU021329; AU043350; AW146323; BUB3; BUB3L; C78067; hBUB3
UniProt ID: (Human) O43684, (Mouse) Q9WVA3
Entrez Gene ID: (Human) 9184, (Mouse) 12237, (Rat) 361662
mitotic sister chromatid segregation
sister chromatid cohesion
mitotic spindle assembly checkpoint
attachment of spindle microtubules to kinetochore
anaphase-promoting complex-dependent catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
cell division
meiotic cell cycle
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
spindle assembly checkpoint
cell cycle
chromosome segregation
mitotic nuclear division
regulation of chromosome segregation
